An appraisal of ultramicrotomy, FIBSEM and cryogenic FIBSEM techniques for the sectioning of biological cells on titanium substrates for TEM investigation

被引：28

作者：

Edwards, H. K. ^{[1
]}

Fay, M. W. ^{[2
]}

Anderson, S. I. ^{[3
]}

Scotchford, C. A. ^{[1
]}

Grant, D. M. ^{[1
]}

Brown, P. D. ^{[1
]}

机构：

[1] Univ Nottingham, Dept Mech Mat & Mfg Engn, Nottingham NG7 2RD, England

[2] Univ Nottingham, Nottingham Nanotechnol & Nanosci Ctr, Nottingham NG7 2RD, England

[3] Univ Nottingham, Queens Med Ctr, Sch Biomed Sci, Nottingham NG7 2RD, England

来源：

JOURNAL OF MICROSCOPY | 2009年 / 234卷 / 01期

基金：

英国工程与自然科学研究理事会;

关键词：

Biomaterial; cryogenic FIBSEM; FIBSEM; human osteoblast; mesenchymal stem cell; titanium; FOCUSED-ION-BEAM; TRANSMISSION ELECTRON-MICROSCOPY; FOCAL ADHESION SITES; SPECIMEN PREPARATION; BONE; INTERFACES; BEHAVIOR; BIOCOMPATIBILITY; IMPLANTS; ALLOYS;

D O I：

10.1111/j.1365-2818.2009.03152.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Ultramicrotomy, focused ion beam scanning electron microscopy (FIBSEM) and cryogenic FIBSEM (cryo-FIBSEM) techniques, as developed for the controlled cross-sectioning of mesenchymal stem cells (MSCs) and human osteoblasts (HObs) on titanium (Ti) substrates for transmission electron microscopy (TEM) investigation, are compared. Conventional ultramicrotomy has been used to section cells on Ti-foil substrates embedded in resin, but significant problems with cell detachment using this technique restricted its general applicability. Conventional FIBSEM 'lift-out' procedures were found to be effective for the preparation of uniform sections of fixed and dehydrated cell/Ti specimens, but the control of cell staining remains an issue. Cryo-FIBSEM procedures used with an 'H-bar' sample geometry enabled the sectioning of fixed and hydrated cell/Ti specimens, but issues remain over ion beam-induced artefacts and control of frost on the sample foils.

引用

页码：16 / 25

页数：10

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