Cadmium inhibits human DNA mismatch repair in vivo

被引：68

作者：

Lützen, A ^{[1
]}

Liberti, SE ^{[1
]}

Rasmussen, LJ ^{[1
]}

机构：

[1] Roskilde Univ, Dept Chem & Life Sci, DK-4000 Roskilde, Denmark

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 321卷 / 01期

关键词：

hEXO1; hMSH2; DNA mismatch repair; cancer; cadmium;

D O I：

10.1016/j.bbrc.2004.06.102

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The heavy metal cadmium (Cd) is a human carcinogen that inhibits DNA repairs activities. We show that DNA mismatch repair (MMR)-mediated cell cycle arrest after alkylation damage is suppressed by exposure to Cd and that this effect is reserved by preincubation with excess of zinc (Zn). We show that Cd-mediated inactivation of MMr activity is not caused by disruption of complex formation between the MMR proteins hEXO1-hMutSalpha and hEXO1-hMutSalpha nor does Cd inhibit 5'-exonuclease activity of hEXO1 in vitro. Thus, our studies show that exposure of human cells to Cd suppresses MMR activity, a repair known to play an important role in colon cancer and that this effect can be reversed by Zn treatment. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：21 / 25

页数：5

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