Chiral separation of cefadroxil by capillary electrochromatography

In this paper, the chiral separation of cefadroxil was studied by capillary electrochromatography. Monolithic capillary column was prepared for the separation of cefadroxil enantiomers. The optimum buffer contained 28.5 mmol/L sodium acetate, 0.95% (v/v) acetic acid, 19 mmol/L beta-cyclodextrin (beta-CD) and 5% (v/v) isopropanol in formamide solution (pH 7.0), with the running voltage of 12 kV, the UV detector wavelength of 254 nm, the sample injected time of 8 s and the temperature of 25 degrees C. Under these conditions, the column efficiency of cefadroxil enantiomers were N-1 = 5324 and N-2 = 23,768 with a selectivity factor (alpha) of 1.056 and resolution (R-s) of 0.978. The effect of buffer pH value, beta-CD concentration, organic modifier (isopropanol) concentration and voltage was also investigated for the separation by CEC. (c) 2006 Elsevier B.V. All rights reserved.