Multichannel homogeneous immunoassay for detection of 2,4,6-trinitrotoluene (TNT) using a microfabricated capillary array electrophoresis chip

被引：60

作者：

Bromberg, A ^{[1
]}

Mathies, RA ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

来源：

ELECTROPHORESIS | 2004年 / 25卷 / 12期

关键词：

capillary array electrophoresis immunoassay; miniaturization; 2,4,6-trinitrotoluene;

D O I：

10.1002/elps.200305818

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-throughput homogeneous immunoassay for the sensitive detection of 2,4,6-tri-nitrotoluene (TNT) has been developed using radial capillary array electrophoresis microdevices. Samples consisting of equilibrium mixtures of anti-TNT antibody (Ab), fluorescein-labeled TNT, and various concentrations of unlabeled TNT were electrokinetically injected into 48 channels of a radial capillary array electrophoresis microchannel plate. The rapid electrophoretic separation allows us to analyze the equilibrium ratio formed by the competition between the labeled and the unlabeled TNT for Ab binding. The simultaneous parallel TNT separations facilitate determination of a calibration curve for the TNT assay, which has high sensitivity (LOD, 1 ng/mL) and a wide dynamic range (1-300 ng/mL).

引用

页码：1895 / 1900

页数：6

共 29 条

[1] Protein microchips: Use for immunoassay and enzymatic reactions [J].

Arenkov, P ;

Kukhtin, A ;

Gemmell, A ;

Voloshchuk, S ;

Chupeeva, V ;

Mirzabekov, A .

ANALYTICAL BIOCHEMISTRY, 2000, 278 (02) :123-131

[2] Micromosaic immunoassays [J].

Bernard, A ;

Michel, B ;

Delamarche, E .

ANALYTICAL CHEMISTRY, 2001, 73 (01) :8-12

[3] Homogeneous immunoassay for detection of TNT and its analogues on a microfabricated capillary electrophoresis chip [J].

Bromberg, A ;

Mathies, RA .

ANALYTICAL CHEMISTRY, 2003, 75 (05) :1188-1195

[4] Analysis of urinary drugs of abuse by a multianalyte capillary electrophoretic immunoassay [J].

Caslavska, J ;

Allemann, D ;

Thormann, W .

JOURNAL OF CHROMATOGRAPHY A, 1999, 838 (1-2) :197-211

[5]

CHEN FTA, 1994, CLIN CHEM, V40, P1819

[6] Development of a multichannel microfluidic analysis system employing affinity capillary electrophoresis for immunoassay [J].

Cheng, SB ;

Skinner, CD ;

Taylor, J ;

Attiya, S ;

Lee, WE ;

Picelli, G ;

Harrison, DJ .

ANALYTICAL CHEMISTRY, 2001, 73 (07) :1472-1479

[7] Microchip-based capillary electrophoresis for immunoassays: Analysis of monoclonal antibodies and theophylline [J].

Chiem, N ;

Harrison, DJ .

ANALYTICAL CHEMISTRY, 1997, 69 (03) :373-378

[8]

Chiem NH, 1998, CLIN CHEM, V44, P591

[9] Monoclonal antibody binding affinity determined by microchip-based capillary electrophoresis [J].

Chiem, NH ;

Harrison, DJ .

ELECTROPHORESIS, 1998, 19 (16-17) :3040-3044

[10] Microfabricated 384-lane capillary array electrophoresis bioanalyzer for ultrahigh-throughput genetic analysis [J].

Emrich, CA ;

Tian, HJ ;

Medintz, IL ;

Mathies, RA .

ANALYTICAL CHEMISTRY, 2002, 74 (19) :5076-5083

← 1 2 3 →