Iodine-assisted matrix-assisted laser desorption/ionisation

被引:7
作者
Bashir, S
Burkitt, WI
Derrick, PJ [1 ]
Giannakopulos, AE
机构
[1] Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England
[2] Univ Warwick, Inst Mass Spectrometry, Coventry CV4 7AL, W Midlands, England
关键词
proteomics; MALDI; matrix;
D O I
10.1016/S1387-3806(02)00749-2
中图分类号
O64 [物理化学(理论化学)、化学物理学]; O56 [分子物理学、原子物理学];
学科分类号
070203 ; 070304 ; 081704 ; 1406 ;
摘要
Addition of iodine to the matrix has been found to lead to lower thresholds for ion formation in matrix-assisted laser desorption/ionisation (MALDI) of peptides and other compounds. Intensities of ions from the matrix have been suppressed through the addition of iodine to the matrix; intensities of ions corresponding to addition of sodium or potassium to the analyte have been diminished. These effects have been observed with a number of matrices, including 2,5-dihydroxybenzoic acid, dithranol and alpha-cyano-4-hydroxy-cinnamic acid.
引用
收藏
页码:697 / 701
页数:5
相关论文
共 40 条
  • [1] Mass spectrometry in proteomics
    Aebersold, R
    Goodlett, DR
    [J]. CHEMICAL REVIEWS, 2001, 101 (02) : 269 - 295
  • [2] Enhanced detection of phosphopeptides in matrix-assisted laser desorption/ionization mass spectrometry using ammonium salts
    Asara, JM
    Allison, J
    [J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1999, 10 (01) : 35 - 44
  • [3] Axelsson J, 1997, RAPID COMMUN MASS SP, V11, P209, DOI 10.1002/(SICI)1097-0231(19970131)11:2<209::AID-RCM734>3.0.CO
  • [4] 2-E
  • [5] Proteomics: new perspectives, new biomedical opportunities
    Banks, RE
    Dunn, MJ
    Hochstrasser, DF
    Sanchez, JC
    Blackstock, W
    Pappin, DJ
    Selby, PJ
    [J]. LANCET, 2000, 356 (9243) : 1749 - 1756
  • [6] BASHIR S, 2001, THESIS U WARWICK
  • [7] HIGH-ACCURACY MOLECULAR MASS DETERMINATION OF PROTEINS USING MATRIX-ASSISTED LASER DESORPTION MASS-SPECTROMETRY
    BEAVIS, RC
    CHAIT, BT
    [J]. ANALYTICAL CHEMISTRY, 1990, 62 (17) : 1836 - 1840
  • [8] TRYPTIC MAPPING OF RECOMBINANT PROTEINS BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY
    BILLECI, TM
    STULTS, JT
    [J]. ANALYTICAL CHEMISTRY, 1993, 65 (13) : 1709 - 1716
  • [9] SUPPRESSION OF MATRIX IONS IN ULTRAVIOLET-LASER DESORPTION - SCANNING ELECTRON-MICROSCOPY AND RAMAN-SPECTROSCOPY OF THE SOLID SAMPLES
    CHAN, TWD
    COLBURN, AW
    DERRICK, PJ
    GARDINER, DJ
    BOWDEN, M
    [J]. ORGANIC MASS SPECTROMETRY, 1992, 27 (03): : 188 - 194
  • [10] MATRIX-ASSISTED LASER DESORPTION IONIZATION USING A LIQUID MATRIX - FORMATION OF HIGH-MASS CLUSTER IONS FROM PROTEINS
    CHAN, TWD
    COLBURN, AW
    DERRICK, PJ
    [J]. ORGANIC MASS SPECTROMETRY, 1992, 27 (01): : 53 - 56