Iodine-assisted matrix-assisted laser desorption/ionisation

被引：7

作者：

Bashir, S

Burkitt, WI

Derrick, PJ ^{[1
]}

Giannakopulos, AE

机构：

[1] Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England

[2] Univ Warwick, Inst Mass Spectrometry, Coventry CV4 7AL, W Midlands, England

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 2002年 / 219卷 / 03期

关键词：

proteomics; MALDI; matrix;

D O I：

10.1016/S1387-3806(02)00749-2

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

Addition of iodine to the matrix has been found to lead to lower thresholds for ion formation in matrix-assisted laser desorption/ionisation (MALDI) of peptides and other compounds. Intensities of ions from the matrix have been suppressed through the addition of iodine to the matrix; intensities of ions corresponding to addition of sodium or potassium to the analyte have been diminished. These effects have been observed with a number of matrices, including 2,5-dihydroxybenzoic acid, dithranol and alpha-cyano-4-hydroxy-cinnamic acid.

引用

页码：697 / 701

页数：5

共 40 条

[31] Salehpour M, 1989, Rapid Commun Mass Spectrom, V3, P259, DOI 10.1002/rcm.1290030804
[32] Talbo G, 1996, RAPID COMMUN MASS SP, V10, P100
[33] Tanaka K, 1988, RAPID COMMUN MASS SP, V2, P151, DOI [10.1002/rcm.1290020802, DOI 10.1002/RCM.1290020802]
[34] Positive ion formation in the ultraviolet matrix-assisted laser desorption/ionization analysis of oligonucleotides by using 2,5-dihydroxybenzoic acid
Tang, W
Nelson, CM
Zhu, L
Smith, LM
[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1997, 8 (03) : 218 - 224
[35] POLY(VINYLIDENE DIFLUORIDE) MEMBRANES AS THE INTERFACE BETWEEN LASER-DESORPTION MASS-SPECTROMETRY, GEL-ELECTROPHORESIS, AND IN-SITU PROTEOLYSIS
VESTLING, MM
FENSELAU, C
[J]. ANALYTICAL CHEMISTRY, 1994, 66 (04) : 471 - 477
[36] IMPROVED MASS ACCURACY IN MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY OF PEPTIDES
VORM, O
MANN, M
[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (11) : 955 - 958
[37] WILM M, 1996, ANAL CHEM, V66, P1
[38] ZENGSTAHL J, 1996, EUR MASS SPECTROM, V2, P23
[39] Zhu YF, 1996, RAPID COMMUN MASS SP, V10, P1591, DOI 10.1002/(SICI)1097-0231(199610)10:13<1591::AID-RCM715>3.0.CO
[40] 2-W

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