Colorimetric screening of bacterial enzyme activity and inhibition based on the aggregation of gold nanoparticles

被引:66
作者
Jiang, Tingting [1 ]
Liu, Rongrong [1 ]
Huang, Xianfeng [1 ]
Feng, Huajun [1 ]
Teo, Weiling [1 ]
Xing, Bengang [1 ]
机构
[1] Nanyang Technol Univ, Div Chem & Biol Chem, Sch Phys & Math Sci, Singapore, Singapore
关键词
BETA-LACTAMASES; ASSAY;
D O I
10.1039/b818853j
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A simple and novel gold nanoparticle (Au-NPs) based colorimetric method has been developed for efficient screening of class A beta-lactamase (Bla) activity and inhibitors in vitro and in bacterial strains.
引用
收藏
页码:1972 / 1974
页数:3
相关论文
共 30 条
[1]   A FUNCTIONAL CLASSIFICATION SCHEME FOR BETA-LACTAMASES AND ITS CORRELATION WITH MOLECULAR-STRUCTURE [J].
BUSH, K ;
JACOBY, GA ;
MEDEIROS, AA .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (06) :1211-1233
[2]   Sensing phosphatase activity by using gold nanoparticles [J].
Choi, Yongdoo ;
Ho, Nan-Hui ;
Tung, Ching-Hsuan .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (05) :707-709
[3]  
de la Fuente JM, 2001, ANGEW CHEM INT EDIT, V40, P2258, DOI 10.1002/1521-3773(20010618)40:12<2257::AID-ANIE2257>3.0.CO
[4]  
2-S
[5]   Gold nanoparticles-based protease assay [J].
Guarise, C ;
Pasquato, L ;
De Filippis, V ;
Scrimin, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (11) :3978-3982
[6]   A broad-spectrum peptide inhibitor of β-lactamase identified using phage display and peptide arrays [J].
Huang, WZ ;
Beharry, Z ;
Zhang, Z ;
Palzkill, T .
PROTEIN ENGINEERING, 2003, 16 (11) :853-860
[7]   Protease-triggered dispersion of nanoparticle assemblies [J].
Laromaine, Anna ;
Koh, Liling ;
Murugesan, Muthu ;
Ulijn, Rein V. ;
Stevens, Molly M. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (14) :4156-+
[8]   Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles [J].
Li, HX ;
Rothberg, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (39) :14036-14039
[9]   A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles [J].
Liu, JW ;
Lu, Y .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (22) :6642-6643
[10]   A simple and specific assay for real-time colorimetric visualization of β-lactamase activity by using gold nanoparticles [J].
Liu, Rongrong ;
Liew, Roushen ;
Zhou, He ;
Xing, Bengang .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (46) :8799-8803