Design and development of heterologous competitive immunoassays for the determination of boscalid residues

被引:12
作者
Esteve-Turrillas, Francesc A. [1 ]
Mercader, Josep V. [1 ]
Agullo, Consuelo [2 ]
Marzo, Javier [2 ]
Abad-Somovilla, Antonio [2 ]
Abad-Fuentes, Antonio [1 ]
机构
[1] Consejo Super Invest Cient IATA CSIC, Inst Agrochem & Food Technol, Valencia 46980, Spain
[2] Univ Valencia, Dept Organ Chem, E-46100 Valencia, Spain
关键词
LINKED IMMUNOSORBENT ASSAYS; PESTICIDES RESIDUES; PICOXYSTROBIN; GENERATION;
D O I
10.1039/c3an01104f
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Boscalid is a modern agrochemical belonging to the so-called chemical class of succinate dehydrogenase inhibitor fungicides. With the aim of developing rapid analytical screening methods for this relevant compound, we herein report the synthesis of new boscalid mimics and the study of their suitability for the production of polyclonal antibodies. Aliphatic spacer arms equivalent in length and composition were tethered at two different aromatic rings of the target molecular structure. These haptens, besides being used for immunization, were employed in the development of heterologous competitive enzyme-linked immunosorbent assays (cELISAs) in order to improve assay detectability. Direct and indirect immunoassays were tailored and applied to the determination of samples with incurred boscalid residues. The assays were characterized in terms of sensitivity, specificity, trueness, and precision. Limit of quantification was established at 5 mu g kg(-1), coefficients of variation were lower than 20%, and recoveries from spiked samples ranged from 90 to 137%. Finally, ELISA performance was evaluated by Deming regression analysis with tomato and cucumber samples, selecting ultra-performance liquid chromatography-mass spectrometry as the reference method. The results showed that the proposed cELISAs are useful for the routine determination of boscalid fungicides in foods with high-sample throughput and affordable cost.
引用
收藏
页码:3636 / 3644
页数:9
相关论文
共 23 条
  • [1] Development of competitive enzyme-linked immunosorbent assays for boscalid determination in fruit juices
    Abad-Fuentes, Antonio
    Esteve-Turrillas, Francesc A.
    Agullo, Consuelo
    Abad-Somovilla, Antonio
    Mercader, Josep V.
    [J]. FOOD CHEMISTRY, 2012, 135 (01) : 276 - 284
  • [2] Immunoassay for Monitoring Environmental and Human Exposure to the Polybrominated Diphenyl Ether BDE-47
    Ahn, Ki Chang
    Gee, Shirley J.
    Tsai, Hsing-Ju
    Bennett, Deborah
    Nishioka, Marcia G.
    Blum, Arlene
    Fishman, Elana
    Hammock, Bruce D.
    [J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2009, 43 (20) : 7784 - 7790
  • [3] Resistance to boscalid fungicide in Alternaria alternata isolates from pistachio in California
    Avenot, Herve F.
    Michailides, Themis J.
    [J]. PLANT DISEASE, 2007, 91 (10) : 1345 - 1350
  • [4] The 2010 European Union Report on Pesticide Residues in Food
    Brocca, Daniela
    Medina-Pastor, Paula
    Miron, Ileana
    Reich, Hermine
    Triacchini, Giuseppe
    Muller, Erica
    van der Schoor, Caroline
    [J]. EFSA JOURNAL, 2013, 11 (03)
  • [5] Characterization of phenolic content, in vitro biological activity, and pesticide loads of extracts from white grape skins from organic and conventional cultivars
    Corrales, Margarita
    Fernandez, Avelina
    Vizoso Pinto, Maria G.
    Butz, Peter
    Franz, Charles M. A. P.
    Schuele, Eberhard
    Tauscher, Bernhard
    [J]. FOOD AND CHEMICAL TOXICOLOGY, 2010, 48 (12) : 3471 - 3476
  • [6] Hapten synthesis, monoclonal antibody generation, and development of competitive immunoassays for the analysis of picoxystrobin in beer
    Esteve-Turrillas, Francesc A.
    Parra, Javier
    Abad-Fuentes, Antonio
    Agullo, Consuelo
    Abad-Somovilla, Antonio
    Mercader, Josep V.
    [J]. ANALYTICA CHIMICA ACTA, 2010, 682 (1-2) : 93 - 103
  • [7] A GENERAL-METHOD FOR DEVELOPING IMMUNOASSAYS TO CHLOROACETANILIDE HERBICIDES
    FENG, PCC
    HORTON, SR
    SHARP, CR
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1992, 40 (02) : 211 - 214
  • [8] COMPETITIVE-INHIBITION ELISA FOR THE S-TRIAZINE HERBICIDES - ASSAY OPTIMIZATION AND ANTIBODY CHARACTERIZATION
    HARRISON, RO
    GOODROW, MH
    HAMMOCK, BD
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1991, 39 (01) : 122 - 128
  • [9] Development of a new QuEChERS method based on dry ice for the determination of 168 pesticides in paprika using tandem mass spectrometry
    Lee, Sung Woo
    Choi, Jeong-Heui
    Cho, Soon-Kil
    Yu, Hyun-A
    Abd El-Aty, A. M.
    Shim, Jae-Han
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2011, 1218 (28) : 4366 - 4377
  • [10] Lehotay SJ, 2007, J AOAC INT, V90, P485