Determination of mobile phase effect on single-component adsorption isotherm by use of numerical estimation

被引：35

作者：

Antos, D ^{[1
]}

Piatkowski, W ^{[1
]}

Kaczmarski, K ^{[1
]}

机构：

[1] Rzeszow Univ Technol, Dept Chem Engn, Fac Chem, Rzeszow, Poland

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 874卷 / 01期

关键词：

adsorption isotherms; mobile phase composition; band profiles; overload gradient elution; preparative chromatography;

D O I：

10.1016/S0021-9673(00)00082-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Numerical estimation was used to determine adsorption isotherm parameters of a single-component in a normal-phase system. The distribution isotherm of methyl deoxycholate was described between the mobile phase containing hexane, ethyl acetate, methanol with varied concentration and a silica gel adsorbent. The effect of the mobile phase composition on the isotherm parameters and the band profiles was investigated. The results obtained were used to simulate the overload gradient elution. The validity of the method proposed was verified by comparison of the computer simulations with the experimental band profiles. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：1 / 12

页数：12

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