A novel PEG-based solid support enables the synthesis of >50 amino-acid peptide thioesters and the total synthesis of a functional SUMO-1 peptide conjugate

被引:31
作者
Boll, Emmanuelle [1 ]
Drobecq, Herve [1 ]
Ollivier, Nathalie [1 ]
Raibaut, Laurent [1 ]
Desmet, Remi [1 ]
Vicogne, Jerome [1 ]
Melnyk, Oleg [1 ]
机构
[1] Univ Lille Nord France, Inst Pasteur Lille, UMR CNRS 8161, F-59021 Lille, France
关键词
NATIVE CHEMICAL LIGATION; INTRAMOLECULAR N; S-ACYL SHIFT; EXPRESSED PROTEIN LIGATION; BIS(2-SULFANYLETHYL)AMIDO PEPTIDES; STAUDINGER LIGATION; UBIQUITIN CHAINS; PHASE SYNTHESIS; CHEMISTRY; UBIQUITYLATION; DIUBIQUITIN;
D O I
10.1039/c3sc53509f
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A bis(2-sulfanylethyl) amino PEG-based resin enabled the synthesis of large (similar to 50 Aa) SEA or thioester peptides using Fmoc-SPPS. These peptide segments permitted the first total synthesis of a 97 amino-acid long SUMO-1-SEA peptide thioester surrogate and of a functional and reversible SUMO-1 peptide conjugate.
引用
收藏
页码:2017 / 2022
页数:6
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