Experimental characterization of 3D localization techniques for particle-tracking and super-resolution microscopy

被引：106

作者：

Mlodzianoski, Michael J. ^{[4
]}

Juette, Manuel F. ^{[1
,2
,4
]}

Beane, Glen L. ^{[3
]}

Bewersdorf, Joerg ^{[4
]}

机构：

[1] Max Planck Inst Met Res, Dept New Mat & Biosyst, D-70569 Stuttgart, Germany

[2] Heidelberg Univ, Dept Biophys Chem, D-70569 Stuttgart, Germany

[3] Jackson Lab, Dept Computat Sci, Bar Harbor, ME 04609 USA

[4] Jackson Lab, Inst Mol Biophys, Bar Harbor, ME 04609 USA

来源：

OPTICS EXPRESS | 2009年 / 17卷 / 10期

关键词：

SINGLE-MOLECULE; FLUORESCENCE MICROSCOPY; ACCURACY; DYNAMICS;

D O I：

10.1364/OE.17.008264

中图分类号：

O43 [光学];

学科分类号：

070207 [光学];

摘要：

Three-dimensional (3D) particle localization at the nanometer scale plays a central role in 3D particle tracking and 3D localization-based super-resolution microscopy. Here we introduce a localization algorithm that is independent of theoretical models and therefore generally applicable to a large number of experimental realizations. Applying this algorithm and a convertible experimental setup we compare the performance of the two major 3D techniques based on astigmatic distortions and on multiplane detection. In both methods we obtain experimental 3D localization accuracies in agreement with theoretical predictions and characterize the depth dependence of the localization accuracy in detail. (C) 2009 Optical Society of America

引用

页码：8264 / 8277

页数：14

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