DNA vaccination of pigs with open reading frame 1-7 of PRRS virus

被引：44

作者：

Barfoed, AM

Blixenkrone-Moller, M

Jensen, MH

Botner, A

Kamstrup, S

机构：

[1] Danish Inst Food & Vet Res, DK-4771 Kalvehave, Denmark

[2] Royal Vet & Agr Univ, Dept Vet Microbiol, Lab Virol & Immunol, DK-1870 Frederiksberg C, Denmark

来源：

VACCINE | 2004年 / 22卷 / 27-28期

关键词：

DNA vaccination; gene gun; pigs; PRRS virus;

D O I：

10.1016/j.vaccine.2004.03.028

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We cloned all open reading frames of a Danish isolate of porcine reproductive and respiratory syndrome (PRRS) virus in DNA vaccination vectors. Pigs were vaccinated using a gene gun with each single construct (ORF1, ORF2, ORF3, ORF4, ORF5, ORF6, or ORF7) or combinations thereof. Vaccination with ORF7 consistently induced antibodies after three vaccinations, while antibodies were only sporadically detected in the remaining groups. After six vaccinations, all pigs were inoculated with PRRS virus and the post-inoculation antibody response was studied. Pigs vaccinated with ORF1 or ORF4 were primed for antibody response against NSP2 or GP4, respectively. Neutralising antibodies were detected in all pigs, with ORF5 vaccinated pigs showing the highest titres. (C) 2004 Elsevier Ltd. All rights reserved.

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页码：3628 / 3641

页数：14

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