Hydroquinone-O,O'-diacetic acid as a more labile replacement for succinic acid linkers in solid-phase oligonucleotide synthesis

被引：21

作者：

Pon, RT

Yu, SY

机构：

[1] University of Calgary, Dept. of Medical Biochemistry, Calgary

来源：

TETRAHEDRON LETTERS | 1997年 / 38卷 / 19期

关键词：

D O I：

10.1016/S0040-4039(97)00619-9

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Hydroquinone-O,O'-diacetic acid (QDA) can be used to link nucleosides to CPG or polystyrene supports instead of succinic acid. Cleavage of oligodeoxy- or oligoribonucleotides, using ammonium hydroxide, requires only two to five minutes. The QDA linker is stable, easily prepared and does not require any other changes to the reagents, methods, or instrumentation used in automated solid-phase oligonucleotide synthesis. (C) 1997 Elsevier Science Ltd.

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页码：3327 / 3330

页数：4

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