Electrochemiluminescence detection of NADH and ethanol based on partial sulfonation of sol-gel network with gold nanoparticles

被引：51

作者：

Deng, Liu ^{[1
]}

Zhang, Lihua ^{[1
]}

Shang, Li ^{[1
]}

Guo, Shaojun ^{[1
]}

Wen, Dan ^{[1
]}

Wang, Fuan ^{[1
]}

Dong, Shaojun ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2009年 / 24卷 / 07期

基金：

中国国家自然科学基金;

关键词：

Electrochemiluminescence; NADH; Alcohol dehydrogenase; (3-mercaptopropyl)-trimethoxysilane; Sol-gel; Gold nanoparticle; ELECTROGENERATED CHEMILUMINESCENCE; ALCOHOL-DEHYDROGENASE; BIOSENSOR; TRIS(2,2'-BIPYRIDYL)RUTHENIUM(II); ELECTRODE;

D O I：

10.1016/j.bios.2008.10.031

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We developed a stable, sensitive electrochemiluminescence (ECL) biosensor based on the synthesis of a new sol-gel material with the ion-exchange capacity sol-gel to coimmobilize the Ru(bpy)(3)(2+) and enzyme. The partial sulfonated (3-mercaptopropyl)-trimethoxysilane sol-gel (PSSG) film acted as both an ion exchanger for the immobilization of Ru(bpy)(3)(2+) and a matrix to immobilize gold nanoparticles (AuNPs). The AuNPs/PSSG/Ru(bpy)(3)(2+) film modified electrode allowed sensitive the ECL detection of NADH as low as 1 nM. Such an ability of AuNPs/PSSG/Ru(bpy)(3)(2+) film to promote the electron transfer between Ru(bpy)(3)(2+) and the electrode suggested a new, promising biocompatible platform for the development of dehydrogenase-based ECL biosensors. With alcohol dehydrogenase (ADH) as a model, we then constructed an ethanol biosensor, which had a linear range of 5 mu M to 5.2 mM with a detection limit of 12 nM. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：2273 / 2276

页数：4

共 21 条

[1] Novel electrochemical interfaces with a tunable kinetic barrier by self-assembling organically modified silica gel and gold nanoparticles [J].

Bharathi, S ;

Nogami, M ;

Ikeda, S .

LANGMUIR, 2001, 17 (01) :1-4

[2] Gold nanoparticle-modified ITO electrode for electrogenerated chemilumineseence: Well-preserved transparency and highly enhanced activity [J].

Chen, Zuofeng ;

Zu, Yanbing .

LANGMUIR, 2007, 23 (23) :11387-11390

[3] Electrogenerated chemiluminese ethanol biosensor based on carbon nanotube-titania-nafion composite film [J].

Choi, Han Nim ;

Yoon, Sook Hyun ;

Lyu, Young-Ku ;

Lee, Won-Yong .

ELECTROANALYSIS, 2007, 19 (04) :459-465

[4] Versatile immunosensor using CdTe quantum dots as electrochemical and fluorescent labels [J].

Cui, Rongjing ;

Pan, Hong-Cheng ;

Zhu, Jun-Jie ;

Chen, Hong-Yuan .

ANALYTICAL CHEMISTRY, 2007, 79 (22) :8494-8501

[5] Synthesis of PtNPs/AQ/Ru(bpy)32+ colloid and its application as a sensitive solid-state electrochemiluminescence sensor material [J].

Du, Yan ;

Qi, Bin ;

Yang, Xiurong ;

Wang, Erkang .

JOURNAL OF PHYSICAL CHEMISTRY B, 2006, 110 (43) :21662-21666

[6] CARBON-SUPPORTED SULFIDE CATALYSTS [J].

DUCHET, JC ;

VANOERS, EM ;

DEBEER, VHJ ;

PRINS, R .

JOURNAL OF CATALYSIS, 1983, 80 (02) :386-402

[7] Characterization of nano-sized CdS-Ag2S core-shell nanoparticles using XPS technique [J].

Hota, G. ;

Idage, S. B. ;

Khilar, Kartic C. .

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2007, 293 (1-3) :5-12

[8] Electrochemiluminescence-based quantitation of classical clinical chemistry analytes [J].

Jameison, F ;

Sanchez, RI ;

Dong, LW ;

Leland, JK ;

Yost, D ;

Martin, MT .

ANALYTICAL CHEMISTRY, 1996, 68 (08) :1298-1302

[9] Gold nanoelectrode ensembles for the simultaneous electrochemical detection of ultratrace arsenic, mercury, and copper [J].

Jena, Bikash Kumar ;

Raj, C. Retna .

ANALYTICAL CHEMISTRY, 2008, 80 (13) :4836-4844

[10] A method to construct a third-generation horseradish peroxidase biosensor: Self-assembling gold nanoparticles to three-dimensional sol-gel network [J].

Jia, JB ;

Wang, BQ ;

Wu, AG ;

Cheng, GJ ;

Li, Z ;

Dong, SJ .

ANALYTICAL CHEMISTRY, 2002, 74 (09) :2217-2223

← 1 2 3 →