Microdissection of Shoot Meristem Functional Domains

被引：61

作者：

Brooks, Lionel, III ^{[1
]}

Strable, Josh ^{[1
]}

Zhang, Xiaolan ^{[2
]}

Ohtsu, Kazuhiro ^{[3
]}

Zhou, Ruilian ^{[3
]}

Sarkar, Ananda ^{[4
]}

Hargreaves, Sarah ^{[3
]}

Elshire, Robert J. ^{[1
]}

Eudy, Douglas ^{[5
]}

Pawlowska, Teresa ^{[6
]}

Ware, Doreen ^{[4
,7
]}

Janick-Buckner, Diane ^{[5
]}

Buckner, Brent ^{[5
]}

Timmermans, Marja C. P. ^{[4
]}

Schnable, Patrick S. ^{[3
]}

Nettleton, Dan ^{[8
]}

Scanlon, Michael J. ^{[1
]}

机构：

[1] Cornell Univ, Dept Plant Biol, Ithaca, NY USA

[2] Univ Georgia, Dept Plant Biol, Athens, GA 30602 USA

[3] Iowa State Univ, Ctr Plant Genom, Ames, IA USA

[4] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

[5] Truman State Univ, Div Sci, Kirksville, MO USA

[6] Cornell Univ, Dept Plant Pathol, Ithaca, NY 14853 USA

[7] USDA, Agr Res Serv Dept, Washington, DC 20250 USA

[8] Iowa State Univ, Dept Stat, Ames, IA USA

来源：

PLOS GENETICS | 2009年 / 5卷 / 05期

基金：

美国国家科学基金会;

关键词：

POLAR AUXIN TRANSPORT; LASER-CAPTURE MICRODISSECTION; GLOBAL GENE-EXPRESSION; MAIZE HOMEOBOX GENE; D-TYPE-CYCLINS; LEAF DEVELOPMENT; APICAL MERISTEM; CELL-DIVISION; ROUGH-SHEATH2; GENE; DOMINANT MUTATION;

D O I：

10.1371/journal.pgen.1000476

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The shoot apical meristem (SAM) maintains a pool of indeterminate cells within the SAM proper, while lateral organs are initiated from the SAM periphery. Laser microdissection-microarray technology was used to compare transcriptional profiles within these SAM domains to identify novel maize genes that function during leaf development. Nine hundred and sixty-two differentially expressed maize genes were detected; control genes known to be upregulated in the initiating leaf (P0/P1) or in the SAM proper verified the precision of the microdissections. Genes involved in cell division/growth, cell wall biosynthesis, chromatin remodeling, RNA binding, and translation are especially upregulated in initiating leaves, whereas genes functioning during protein fate and DNA repair are more abundant in the SAM proper. In situ hybridization analyses confirmed the expression patterns of six previously uncharacterized maize genes upregulated in the P0/P1. P0/P1-upregulated genes that were also shown to be downregulated in leaf-arrested shoots treated with an auxin transport inhibitor are especially implicated to function during early events in maize leaf initiation. Reverse genetic analyses of asceapen1 (asc1), a maize D4-cyclin gene upregulated in the P0/P1, revealed novel leaf phenotypes, less genetic redundancy, and expanded D4-CYCLIN function during maize shoot development as compared to Arabidopsis. These analyses generated a unique SAM domain-specific database that provides new insight into SAM function and a useful platform for reverse genetic analyses of shoot development in maize.

引用

页数：14

共 91 条

[1]

BAUMBERGER N, 2005, PLANT CELL, V14, P3029

[2] CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].