Detection of Sclerotinia sclerotiorum in Planta by a Real-time PCR Assay

被引：25

作者：

Yin, Yanni ^{[1
]}

Ding, Laisong ^{[1
]}

Liu, Xin ^{[1
]}

Yang, Jinghui ^{[1
]}

Ma, Zhonghua ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Biotechnol, Hangzhou 310029, Zhejiang, Peoples R China

来源：

JOURNAL OF PHYTOPATHOLOGY | 2009年 / 157卷 / 7-8期

关键词：

oilseed rape; real-time polymerase chain reaction; Sclerotinia sclerotiorum; STEM ROT; PATHOGEN; CANOLA;

D O I：

10.1111/j.1439-0434.2009.01543.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Stem rot caused by Sclerotinia sclerotiorum is a very serious disease on oilseed rape worldwide. In this study, a pair of polymerase chain reaction (PCR) primers was designed based on the nucleotide sequence of a DNA region amplified by a microsatellite primer M13. The primer pair amplified a 252-bp fragment from all S. sclerotiorum isolates collected from oilseed rapes at different locations in different years, but not from any other fungus tested. Using this pair of primers, a real-time PCR assay was developed to rapidly detect early infection of S. sclerotiorum on petals of oilseed rape. The real-time PCR assay developed in this study could help growers make a timely decision on fungicide application.

引用

页码：465 / 469

页数：5

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