Impedimetric genosensors for the detection of DNA hybridization

被引：59

作者：

Bonanni, A. ^{[1
]}

Esplandiu, M. J. ^{[1
]}

Pividori, M. I. ^{[1
]}

Alegret, S. ^{[1
]}

del Valle, M. ^{[1
]}

机构：

[1] Univ Autonoma Barcelona, Dept Chem, Sensors & Biosensors Grp, E-08193 Barcelona, Spain

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2006年 / 385卷 / 07期

关键词：

DNA biosensor; impedance; capacitance; unlabelled DNA;

D O I：

10.1007/s00216-006-0558-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Impedance spectroscopy is proposed as the transduction principle for detecting the hybridization of DNA complementary strands. In our experiments, different DNA oligonucleotides were used as model gene substances. The gene probe is first immobilized on a graphite-epoxy composite working electrode based genosensor. Detection principle is based on changes of impedance spectra of a redox marker, the ferro/ferricyanide couple, after hybridization with target DNA. Resistance offered to the electrochemical reaction serves as the working signal, allowing for an unlabelled gene assay.

引用

页码：1195 / 1201

页数：7

共 37 条

[21] Toward an ICPMS-linked DNA assay based on gold nanoparticles immunoconnected through peptide sequences
Merkoçi, A
Aldavert, M
Tarrasón, G
Eritja, R
Alegret, S
[J]. ANALYTICAL CHEMISTRY, 2005, 77 (19) : 6500 - 6503
[22] New materials for electrochemical sensing V:: Nanoparticles for DNA labeling
Merkoçi, A
Aldavert, M
Marín, S
Alegret, S
[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2005, 24 (04) : 341 - 349
[23] Mickelsen SR, 1996, ELECTROANAL, V8, P15
[24] VOLTAMMETRIC DNA BIOSENSOR FOR CYSTIC-FIBROSIS BASED ON A MODIFIED CARBON-PASTE ELECTRODE
MILLAN, KM
SARAULLO, A
MIKKELSEN, SR
[J]. ANALYTICAL CHEMISTRY, 1994, 66 (18) : 2943 - 2948
[25] Photoswitchable antigen-antibody interactions studied by impedance spectroscopy
Patolsky, F
Filanovsky, B
Katz, E
Willner, I
[J]. JOURNAL OF PHYSICAL CHEMISTRY B, 1998, 102 (50) : 10359 - 10367
[26] PENG H, 2005, ELECTROCHIM ACTA, V51, P1376
[27] Study of the DNA hybridization transduction behavior of a quinone-containing electroactive polymer by cyclic voltammetry and electrochemical impedance spectroscopy
Piro, B
Haccoun, J
Pham, MC
Tran, LD
Rubin, A
Perrot, H
Gabrielli, C
[J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2005, 577 (01) : 155 - 165
[28] FIBEROPTIC DNA SENSOR FOR FLUOROMETRIC NUCLEI ACID DETERMINATION
PIUNNO, PAE
KRULL, UJ
HUDSON, RHE
DAMHA, MJ
COHEN, H
[J]. ANALYTICAL CHEMISTRY, 1995, 67 (15) : 2635 - 2643
[29] Graphite-epoxy composites as a new transducing material for electrochemical genosensing
Pividori, MI
Merkoçi, A
Alegret, S
[J]. BIOSENSORS & BIOELECTRONICS, 2003, 19 (05) : 473 - 484
[30] Dot-blot amperometric genosensor for detecting a novel determinant of β-lactamase resistance in Staphylococcus aureus
Pividori, MI
Merkoçi, A
Alegret, S
[J]. ANALYST, 2001, 126 (09) : 1551 - 1557

← 1 2 3 4 →