Highly efficient peptide separations in proteomics Part 2: Bi- and multidimensional liquid-based separation techniques

被引：81

作者：

Sandra, Koen ^{[1
,2
]}

Moshir, Mahan ^{[2
]}

D'hondt, Filip ^{[2
]}

Tuytten, Robin ^{[2
]}

Verleysen, Katleen ^{[2
]}

Kas, Koen ^{[2
]}

Francois, Isabelle ^{[3
]}

Sandra, Pat ^{[1
,3
]}

机构：

[1] RIC, B-8500 Kortrijk, Belgium

[2] VIB Bio Incubator, B-9052 Zwijnaarde Ghent, Belgium

[3] Univ Ghent, Dept Organ Chem, B-9000 Ghent, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 11-12期

关键词：

Proteomics; Peptides; Multidimensional; Chromatography; Electrophoresis; On-line; Off-line; TANDEM MASS-SPECTROMETRY; HYDROPHILIC-INTERACTION CHROMATOGRAPHY; PROTEIN IDENTIFICATION TECHNOLOGY; CATION-EXCHANGE CHROMATOGRAPHY; LARGE-SCALE IDENTIFICATION; REVERSED-PHASE HPLC; PEAK-CAPACITY; ION-EXCHANGE; OFF-LINE; 2-DIMENSIONAL ELECTROPHORESIS;

D O I：

10.1016/j.jchromb.2009.02.050

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Multidimensional liquid-based separation techniques are described for maximizing the resolution of the enormous number of peptides generated upon tryptic digestion of proteomes, and hence, reduce the spatial and temporal complexity of the sample to a level that allows successful mass spectrometric analysis. This review complements the previous contribution on unidimensional high performance liquid chromatography (HPLC). Both chromatography and electrophoresis will be discussed albeit with reversed-phase HPLC (RPLC) as the final separation dimension prior to MS analysis. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1019 / 1039

页数：21

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