Evaluation of a novel vesicular stomatitis virus pseudotype-based assay for detection of neutralizing antibody responses to SARS-CoV

被引：33

作者：

Fukushi, Shuetsu

Mizutani, Tetsuya

Saijo, Masayuki

Kurane, Ichiro

Taguchi, Fumihiro

Tashiro, Masato

Morikawa, Shigeru

机构：

[1] Natl Inst Infect Dis, Special Pathogens Lab, Dept Virol 1, Tokyo, Japan

[2] Natl Inst Infect Dis, Dept Virol 3, Tokyo, Japan

来源：

JOURNAL OF MEDICAL VIROLOGY | 2006年 / 78卷 / 12期

关键词：

SARS coronavirus; VSV; pseudotype; neutralization;

D O I：

10.1002/jmv.20732

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Severe acute respiratory syndrome (SARS)-coronavirus (SARS-CoV) is the causative agent of SARS. The S protein of SARS-CoV is a major target for neutralizing antibodies (Nabs) in infected patients. We developed a neutralization assay using a recombinant vesicular stomatitis virus (VSV) bearing SARS-CoV-S protein (VSV-SARS-St19). A total of 56 serum samples collected from 22 healthcare workers in the Hanoi French Hospital during the SARS epidemic in 2003 were evaluated and compared to the conventional neutralizing assay using infectious SARS-CoV. The results of the neutralization assay using VSV-SARS-St19 pseudotype showed good correlations with those using infectious SARS-CoV. The newly developed neutralization assay was more sensitive to low antibody titers in serum samples. Thus, the VSV-SARS-St19 is a useful tool for detecting Nabs against SARS-CoV.

引用

收藏

页码：1509 / 1512

页数：4

相关论文

共 11 条

[1] Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein [J].

Fukushi, S ;

Mizutani, T ;

Saijo, M ;

Matsuyama, S ;

Miyajima, N ;

Taguchi, F ;

Itamura, S ;

Kurane, I ;

Morikawa, S .

JOURNAL OF GENERAL VIROLOGY, 2005, 86 :2269-2274

[2] Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor [J].

Hofmann, H ;

Geier, M ;

Marzi, A ;

Krumbiegel, M ;

Peipp, M ;

Fey, GH ;

Gramberg, T ;

Pöhlmann, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 319 (04) :1216-1221

[3] Comparison of two real-time quantitative assays for detection of severe acute respiratory syndrome coronavirus [J].

Hourfar, MK ;

Roth, WK ;

Seifried, E ;

Schmidt, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (05) :2094-2100

[4] Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus [J].

Li, WH ;

Moore, MJ ;

Vasilieva, N ;

Sui, JH ;

Wong, SK ;

Berne, MA ;

Somasundaran, M ;

Sullivan, JL ;

Luzuriaga, K ;

Greenough, TC ;

Choe, H ;

Farzan, M .

NATURE, 2003, 426 (6965) :450-454

[5] Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expression [J].

Nie, YC ;

Wang, PG ;

Shi, XL ;

Wang, GW ;

Chen, J ;

Zheng, AH ;

Wang, W ;

Wang, Z ;

Qu, XX ;

Luo, M ;

Tan, L ;

Song, XJ ;

Yin, XL ;

Chen, JG ;

Ding, MX ;

Deng, HK .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 321 (04) :994-1000

[6] Use of vesicular stomatitis virus pseudotypes bearing hantaan or Seoul virus envelope proteins in a rapid and safe neutralization test [J].

Ogino, M ;

Ebihara, H ;

Lee, BH ;

Araki, K ;

Lundkvist, Å ;

Kawaoka, Y ;

Yoshimatsu, K ;

Arikawa, J .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2003, 10 (01) :154-160

[7] Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS [J].

Saijo, M ;

Ogino, T ;

Taguchi, F ;

Fukushi, S ;

Mizutani, T ;

Notomi, T ;

Kanda, H ;

Minekawa, H ;

Matsuyama, S ;

Long, HT ;

Hanh, NTH ;

Kurane, I ;

Tashiro, M ;

Morikawa, S .

JOURNAL OF VIROLOGICAL METHODS, 2005, 125 (02) :181-186

[8] A system for functional analysis of Ebola virus glycoprotein [J].

Takada, A ;

Robison, C ;

Goto, H ;

Sanchez, A ;

Murti, KG ;

Whitt, MA ;

Kawaoka, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (26) :14764-14769

[9] Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes [J].

Temperton, NJ ;

Chan, PK ;

Simmons, G ;

Zambon, MC ;

Tedder, RS ;

Takeuchi, Y ;

Weiss, RA .

EMERGING INFECTIOUS DISEASES, 2005, 11 (03) :411-416

[10] Development and evaluation of a novel loop-mediated isothermal amplification method for rapid detection of severe acute respiratory syndrome coronavirus [J].

Thai, HTC ;

Le, MQ ;

Vuong, CD ;

Parida, M ;

Minekawa, H ;

Notomi, T ;

Hasebe, F ;

Morita, K .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (05) :1956-1961