Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae

被引：519

作者：

Wieczorke, R ^{[1
]}

Krampe, S ^{[1
]}

Weierstall, T ^{[1
]}

Freidel, K ^{[1
]}

Hollenberg, CP ^{[1
]}

Boles, E ^{[1
]}

机构：

[1] Univ Dusseldorf, Inst Mikrobiol, D-40225 Dusseldorf, Germany

来源：

FEBS LETTERS | 1999年 / 464卷 / 03期

关键词：

functional analysis; gene family; sugar transport; multiple deletion; yeast; gene redundancy;

D O I：

10.1016/S0014-5793(99)01698-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hexose transporter family of Saccharomyces cerevisiae comprises 18 proteins (Hxt1-17, Gal2), Here, we demonstrate that all these proteins, except Hxt12, and additionally three members of the maltose transporter family (Agt1, Yd1247, Yjr160) are able to transport hexoses, In a yeast strain deleted for HXT1-17, GAL2, AGT1, YDL247w and YJR160c, glucose consumption and transport activity were completely abolished. However, as additional deletion of the glucose sensor gene SNF3 partially restored growth on hexoses, our data indicate the existence of even more proteins able to transport hexoses in yeast. (C) 1999 Federation of European Biochemical Societies.

引用

页码：123 / 128

页数：6

共 29 条

[1] An overview of membrane transport proteins in Saccharomyces cerevisiae [J].

Andre, B .

YEAST, 1995, 11 (16) :1575-1611

[2] MAMMALIAN PASSIVE GLUCOSE TRANSPORTERS - MEMBERS OF AN UBIQUITOUS FAMILY OF ACTIVE AND PASSIVE TRANSPORT PROTEINS [J].

BALDWIN, SA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1154 (01) :17-49

[3] Identification and characterization of MAE1, the Saccharomyces cerevisiae structural gene encoding mitochondrial malic enzyme [J].

Boles, E ;

de Jong-Gubbels, P ;

Pronk, JT .

JOURNAL OF BACTERIOLOGY, 1998, 180 (11) :2875-2882

[4] SACCHAROMYCES-CEREVISIAE PHOSPHOGLUCOSE ISOMERASE AND FRUCTOSE BISPHOSPHATE ALDOLASE CAN BE REPLACED FUNCTIONALLY BY THE CORRESPONDING ENZYMES OF ESCHERICHIA-COLI AND DROSOPHILA-MELANOGASTER [J].

BOLES, E ;

ZIMMERMANN, FK .

CURRENT GENETICS, 1993, 23 (03) :187-191

[5] Cloning of a second gene encoding 6-phosphofructo-2-kinase in yeast, and characterization of mutant strains without fructose-2,6-bisphosphate [J].

Boles, E ;

Gohlmann, HWH ;

Zimmerman, FK .

MOLECULAR MICROBIOLOGY, 1996, 20 (01) :65-76

[6] The molecular genetics of hexose transport in yeasts [J].

Boles, E ;

Hollenberg, CP .

FEMS MICROBIOLOGY REVIEWS, 1997, 21 (01) :85-111

[7] The transcriptional program of sporulation in budding yeast [J].

Chu, S ;

DeRisi, J ;

Eisen, M ;

Mulholland, J ;

Botstein, D ;

Brown, PO ;

Herskowitz, I .

SCIENCE, 1998, 282 (5389) :699-705

[8] Exploring the metabolic and genetic control of gene expression on a genomic scale [J].

DeRisi, JL ;

Iyer, VR ;

Brown, PO .

SCIENCE, 1997, 278 (5338) :680-686

[9] AN EFFICIENT TRANSFORMATION PROCEDURE ENABLING LONG-TERM STORAGE OF COMPETENT CELLS OF VARIOUS YEAST GENERA [J].

DOHMEN, RJ ;

STRASSER, AWM ;

HONER, CB ;

HOLLENBERG, CP .

YEAST, 1991, 7 (07) :691-692

[10] A new efficient gene disruption cassette for repeated use in budding yeast [J].

Guldener, U ;

Heck, S ;

Fiedler, T ;

Beinhauer, J ;

Hegemann, JH .

NUCLEIC ACIDS RESEARCH, 1996, 24 (13) :2519-2524

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