Detection of plant viruses using a surface plasmon resonance via complexing with specific antibodies

被引:53
作者
Boltovets, PM
Snopok, BA
Boyko, VR
Shevchenko, TP
Dyachenko, NS
Shirshov, YM
机构
[1] Natl Acad Sci, DK Zabolotny Inst Microbiol & Virol, UA-03143 Kiev, Ukraine
[2] Natl Acad Sci Ukraine, Inst Semicond Phys, UA-03028 Kiev, Ukraine
[3] Taras Shevchenko Natl Univ, UA-03022 Kiev, Ukraine
关键词
rapid diagnosis; intact viruses; tobacco mosaic virus; surface plasmon resonance;
D O I
10.1016/j.jviromet.2004.06.019
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The use of instrumental systems based on the surface plasmon resonance (SPR) for rapid diagnosis of intact plant viruses (in particular, tobacco mosaic virus (TMV)) is considered. A new approach using detection of viral antigen and antibody (IgG) complexes formed during the preincubation step (instead of their consecutive application in classical approach) is discussed. A comparison between signal level registered from the mixture of virus and specific serum and that from the sample without virus (samples deposited onto the sensor surface treated with thiocyanate and protein A Staphylococcus aureus) allows unambiguous detection of viral particles in the material studied. The performance capabilities of the method are discussed and illustrated by quantitative detection of virus in the actual samples (cells homogenate) at high concentration. 0 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:101 / 106
页数:6
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