Bioceramic-collagen scaffolds loaded with human adipose-tissue derived stem cells for bone tissue engineering

被引：52

作者：

Daei-farshbaf, Neda ^{[1
,2
]}

Ardeshirylajimi, Abdolreza ^{[3
]}

Seyedjafari, Ehsan ^{[4
]}

Piryaei, Abbas ^{[1
,2
]}

Fathabady, Fatemeh Fadaei ^{[1
,2
]}

Hedayati, Mehdi ^{[5
]}

Salehi, Mohammad ^{[6
]}

Soleimani, Masoud ^{[7
]}

Nazarian, Hamid ^{[1
,2
]}

Moradi, Sadegh-Lotfalah ^{[3
]}

Norouzian, Mohsen ^{[1
,2
]}

机构：

[1] Shahid Beheshti Univ Med Sci, Dept Anat, Tehran, Iran

[2] Shahid Beheshti Univ Med Sci, Dept Cell Biol, Tehran, Iran

[3] Stem Cell Technol Res Ctr, Dept Stem Cell Biol, Tehran, Iran

[4] Univ Tehran, Dept Biotechnol, Coll Sci, Tehran, Iran

[5] Shahid Beheshti Univ Med Sci, Endocrine Res Ctr, Tehran, Iran

[6] Shahid Beheshti Univ Med Sci, Dept Biotechnol, Fac Med, Tehran, Iran

[7] Tarbiat Modares Univ, Dept Hematol, Fac Med Sci, Tehran, Iran

来源：

MOLECULAR BIOLOGY REPORTS | 2014年 / 41卷 / 02期

关键词：

Mesenchymal stem cells; Tissue engineering; Bone; Bioceramic; Critical-size defect; THYROID-HORMONES; REPAIR; HYDROXYAPATITE; DEFECTS; DIFFERENTIATION; HYPOTHYROIDISM; REGENERATION; NANOFIBER;

D O I：

10.1007/s11033-013-2913-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The combination of bioceramics and stem cells has attracted the interest of research community for bone tissue engineering applications. In the present study, a combination of Bio-Oss(A (R)) and type 1 collagen gel as scaffold were loaded with human adipose-tissue derived mesenchymal stem cells (AT-MSCs) after isolation and characterization, and the capacity of them for bone regeneration was investigated in rat critical size defects using digital mammography, multi-slice spiral computed tomography imaging and histological analysis. 8 weeks after implantation, no mortality or sign of inflammation was observed in the site of defect. According to the results of imaging analysis, a higher level of bone regeneration was observed in the rats receiving Bio-Oss(A (R))-Gel compared to untreated group. In addition, MSC-seeded Bio-Oss-Gel induced the highest bone reconstruction among all groups. Histological staining confirmed these findings and impressive osseointegration was observed in MSC-seeded Bio-Oss-Gel compared with Bio-Oss-Gel. On the whole, it was demonstrated that combination of AT-MSCs, Bio-Oss and Gel synergistically enhanced bone regeneration and reconstruction and also could serve as an appropriate structure to bone regenerative medicine and tissue engineering application.

引用

页码：741 / 749

页数：9

共 32 条

[1]

TSH is a negative regulator of skeletal remodeling [J].

Abe, E ;

Marians, RC ;

Yu, WQ ;

Wu, XB ;

Ando, T ;

Li, YN ;

Iqbal, J ;

Eldeiry, L ;

Rajendren, G ;

Blair, HC ;

Davies, TF ;

Zaidi, M .

CELL, 2003, 115 (02) :151-162

[2]

Dynamics of Bio-Oss® Collagen incorporation in fresh extraction wounds: an experimental study in the dog [J].

Araujo, Mauricio G. ;

Liljenberg, Birgitta ;

Lindhe, Jan .

CLINICAL ORAL IMPLANTS RESEARCH, 2010, 21 (01) :55-64

[3]

Enhanced reconstruction of rat calvarial defects achieved by plasma-treated electrospun scaffolds and induced pluripotent stem cells [J].

Ardeshirylajimi, Abdolreza ;

Dinarvand, Peyman ;

Seyedjafari, Ehsan ;

Langroudi, Lida ;

Adegani, Fatemeh Jamshidi ;

Soleimani, Masoud .

CELL AND TISSUE RESEARCH, 2013, 354 (03) :849-860

[4]

Nanofiber-based polyethersulfone scaffold and efficient differentiation of human induced pluripotent stem cells into osteoblastic lineage [J].

Ardeshirylajimi, Abdolreza ;

Hosseinkhani, Saman ;

Parivar, Kazem ;

Yaghmaie, Parichehr ;

Soleimani, Masoud .

MOLECULAR BIOLOGY REPORTS, 2013, 40 (07) :4287-4294

[5]

Bone regeneration and stem cells [J].

Arvidson, K. ;

Abdallah, B. M. ;

Applegate, L. A. ;

Baldini, N. ;

Cenni, E. ;

Gomez-Barrena, E. ;

Granchi, D. ;

Kassem, M. ;

Konttinen, Y. T. ;

Mustafa, K. ;

Pioletti, D. P. ;

Sillat, T. ;

Finne-Wistrand, A. .

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2011, 15 (04) :718-746

[6]

Asti Annalia, 2008, Technol Health Care, V16, P401

[7]

A lack of thyroid hormones rather than excess thyrotropin causes abnormal skeletal development in hypothyroidism [J].

Bassett, J. H. Duncan ;

Williams, Allan J. ;

Murphy, Elaine ;

Boyde, Alan ;

Howell, Peter G. T. ;

Swinhoe, Rowan ;

Archanco, Marta ;

Flamant, Frederic ;

Samarut, Jacques ;

Costagliola, Sabine ;

Vassart, Gilbert ;

Weiss, Roy E. ;

Refetoff, Samuel ;

Williams, Graham R. .

MOLECULAR ENDOCRINOLOGY, 2008, 22 (02) :501-512

[8]

The response of bone to nanocrystalline hydroxyapatite-coated Ti13Nb11Zr alloy in an animal model [J].

Bigi, Adriana ;

Fini, Milena ;

Bracci, Barbara ;

Boanini, Elisa ;

Torricelli, Paola ;

GiavareSi, Gianluca ;

Aldini, Nicolo N. ;

Facchini, Alessandro ;

Sbaiz, Fausto ;

Giardino, Roberto .

BIOMATERIALS, 2008, 29 (11) :1730-1736

[9]

Repair of cranial bone defects with adipose derived stem cells and coral scaffold in a canine model [J].

Cui, Lei ;

Liu, Bo ;

Liu, Guangpeng ;

Zhang, Wenjie ;

Cen, Lian ;

Sun, Jian ;

Yin, Shuo ;

Liu, Wei ;

Cao, Yilin .

BIOMATERIALS, 2007, 28 (36) :5477-5486

[10]

Novel approach to reduce postsurgical adhesions to a minimum: Administration of losartan plus atorvastatin intraperitoneally [J].

Dinarvand, Peyman ;

Farhadian, Shirin ;

Seyedjafari, Ehsan ;

Shafiee, Abbas ;

Jalali, Arash ;

Sanaei-rad, Parisa ;

Dinarvand, Babak ;

Soleimani, Masoud .

JOURNAL OF SURGICAL RESEARCH, 2013, 181 (01) :91-98

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