High-sensitivity vibrational imaging with frequency modulation coherent anti-Stokes Raman scattering (FM CARS) microscopy

被引：202

作者：

Ganikhanov, Feruz ^{[1
]}

Evans, Conor L. ^{[1
]}

Saar, Brian G. ^{[1
]}

Xie, X. Sunney ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

来源：

OPTICS LETTERS | 2006年 / 31卷 / 12期

关键词：

Imaging techniques;

D O I：

10.1364/OL.31.001872

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate a new approach to coherent anti-Stokes Raman scattering (CARS) microscopy that significantly increases the detection sensitivity. CARS signals are generated by collinearly overlapped, tightly focused, and raster scanned pump and Stokes laser beams, whose difference frequency is rapidly modulated. The resulting amplitude modulation of the CARS signal is detected through a lock-in amplifier. This scheme efficiently suppresses the nonresonant background and allows for the detection of far fewer vibrational oscillators than possible through existing CARS microscopy methods. (c) 2006 Optical Society of America

引用

页码：1872 / 1874

页数：3

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