Conductometric tyrosinase biosensor for the detection of diuron, atrazine and its main metabolites

被引:65
作者
Anh, TM
Dzyadevych, SV
Van, MC
Renault, NJ
Duc, CN
Chovelon, JM
机构
[1] Univ Lyon 1, CNRS, UMR 5634, Lab Applicat Chim Enivronm, F-69622 Villeurbanne, France
[2] Hanoi Univ Technol, ITIMS, Hanoi, Vietnam
[3] Natl Acad Sci Ukraine, Inst Mol Biol & Genet, Lab Biomol Elect, UA-03143 Kiev, Ukraine
[4] Vietnam Natl Ctr Sci & Technol, Inst Nat Prod Chem, Hanoi, Vietnam
[5] Ecole Cent Lyon, FRE 2608, IFoS, F-69134 Ecully, France
关键词
conductometric biosensor; tyrosinase; atrazine; Diuron;
D O I
10.1016/j.talanta.2003.11.008
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The determination of diuron. atrazine, desisopropylatrazine (DIA) and desethylatrazine (DEA) were investigated using conductometric tyrosinase biosensor. Tyrosinase was immobilised on the biosensor sensitive part by allowing it to mix with bovine serum albumin (BSA) and then cross-linking in saturated glutaraldehyde (GA) vapour for 30 min. The determination of pollutants in a solution was performed by comparison of the Output signal (i.e percentage of the enzymatic activity) of the biosensor before and after contact With pollutants. The measurement of the enzymatic activity was performed using 4-chlorophenol, phenol and catechol Substrates and response times ranging from 1 to 5 min were observed. A 4-chlorophenol substrate was used to detect pesticides. A 30 min contact time of the biosensor in the pollutant solution was used. Under the experimental conditions employed, detection limits for diuron and atrazine were about 1 ppb and dynamic range of 2.3-2330 and 2.15-2150 ppb were obtained for diuron and atrazine, respectively. A relative standard deviation (n = 3) of the output signal was estimated to be 5% and a slight drift of 1.5 muS h(-1) was observed. The 90% of the enzyme activity was still maintained after 23 days of storage in a buffer solution at 4 degreesC. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:365 / 370
页数:6
相关论文
共 28 条
[1]   Development of tyrosinase biosensor based on pH-sensitive field-effect transistors for phenols determination in water solutions [J].
Anh, TM ;
Dzyadevych, SV ;
Soldatkin, AP ;
Chien, ND ;
Jaffrezic-Renault, N ;
Chovelon, JM .
TALANTA, 2002, 56 (04) :627-634
[2]   A BIOSENSOR AS WARNING DEVICE FOR THE DETECTION OF CYANIDE, CHLOROPHENOLS, ATRAZINE AND CARBAMATE PESTICIDES [J].
BESOMBES, JL ;
COSNIER, S ;
LABBE, P ;
REVERDY, G .
ANALYTICA CHIMICA ACTA, 1995, 311 (03) :255-263
[3]   DETERMINATION OF PHENOLIC-COMPOUNDS IN SURFACE-WATER USING ONLINE LIQUID-CHROMATOGRAPHIC PRECOLUMN-BASED COLUMN-SWITCHING TECHNIQUES [J].
BROUWER, ER ;
BRINKMAN, UAT .
JOURNAL OF CHROMATOGRAPHY A, 1994, 678 (02) :223-231
[4]   DETERMINATION OF PHENOL IN WASTES AND WATER USING AN ENZYME SENSOR [J].
CAMPANELLA, L ;
BEONE, T ;
SAMMARTINO, MP ;
TOMASSETTI, M .
ANALYST, 1993, 118 (08) :979-986
[5]   COMPARISON OF IMMOBILIZATION PROCEDURES FOR DEVELOPMENT OF AN ELECTROCHEMICAL PPO-BASED BIOSENSOR FOR ON LINE MONITORING OF A DEPURATION PROCESS [J].
CANOFENI, S ;
DISARIO, S ;
MELA, J ;
PILLOTON, R .
ANALYTICAL LETTERS, 1994, 27 (09) :1659-1669
[6]   A NEW STRATEGY FOR THE CONSTRUCTION OF A TYROSINASE-BASED AMPEROMETRIC PHENOL AND O-DIPHENOL SENSOR [J].
COSNIER, S ;
INNOCENT, C .
BIOELECTROCHEMISTRY AND BIOENERGETICS, 1993, 31 (02) :147-160
[7]   Poly(amphiphilic pyrrole)-tyrosinase-peroxidase electrode for amplified flow injection-amperometric detection of phenol [J].
Cosnier, S ;
Popescu, IC .
ANALYTICA CHIMICA ACTA, 1996, 319 (1-2) :145-151
[8]   Application of enzyme field-effect transistors for determination of glucose concentrations in blood serum [J].
Dzyadevich, SV ;
Korpan, YI ;
Arkhipova, VN ;
Alesina, MY ;
Martelet, C ;
El'Skaya, AV ;
Soldatkin, AP .
BIOSENSORS & BIOELECTRONICS, 1999, 14 (03) :283-287
[9]   Development of enzyme biosensor based on pH-sensitive field-effect transistors for detection of phenolic compounds [J].
Dzyadevych, SV ;
Anh, TM ;
Soldatkin, AP ;
Chien, ND ;
Jaffrezic-Renault, N ;
Chovelon, JM .
BIOELECTROCHEMISTRY, 2002, 55 (1-2) :79-81
[10]   Non-aqueous flow-injection determination of atrazine by inhibition of immobilized tyrosinase [J].
Hipólito-Moreno, A ;
León-González, ME ;
Pérez-Arribas, LV ;
Polo-Díez, LM .
ANALYTICA CHIMICA ACTA, 1998, 362 (2-3) :187-192