Cell contact-dependent acquisition of cellular and viral nonautonomously encoded small RNAs

被引：89

作者：

Rechavi, Oded ^{[2
]}

Erlich, Yaniv ^{[1
]}

Amram, Hila ^{[3
,4
]}

Flomenblit, Lena ^{[3
,4
]}

Karginov, Fedor V. ^{[1
]}

Goldstein, Itamar ^{[3
,4
]}

Hannon, Gregory J. ^{[1
]}

Kloog, Yoel ^{[2
]}

机构：

[1] Cold Spring Harbor Lab, Howard Hughes Med Inst, Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA

[2] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Neurobiol, IL-69978 Tel Aviv, Israel

[3] Chaim Sheba Med Ctr, Canc Res Ctr, Program Immunol, IL-52621 Tel Hashomer, Israel

[4] Tel Aviv Univ, Sackler Sch Med, IL-69978 Tel Aviv, Israel

来源：

GENES & DEVELOPMENT | 2009年 / 23卷 / 16期

关键词：

microRNA; shRNA; intercellular transfer; immunity; INTERCELLULAR TRANSFER; IMMUNOLOGICAL SYNAPSE; MICRORNAS; TRANSPORT; MESSENGER; INTERFERENCE; MECHANISM; PROTEINS; GENOMICS;

D O I：

10.1101/gad.1789609

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In some organisms, small RNA pathways can act nonautonomously, with responses spreading from cell to cell. Dedicated intercellular RNA delivery pathways have not yet been characterized in mammals, although secretory compartments have been found to contain RNA. Here we show that, upon cell contact, T cells acquire from B cells small RNAs that can impact the expression of target genes in the recipient T cells. Synthetic microRNA ( miRNA) mimetics, viral miRNAs expressed by infected B cells, and endogenous miRNAs could all be transferred into T cells. These mechanisms may allow small RNA-mediated communication between immune cells. The documented transfer of viral miRNAs raises the possible exploitation of these pathways for viral manipulation of the host immune response.

引用

页码：1971 / 1979

页数：9

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