Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes

被引:48
作者
Kong, Fanrong
Brown, Mitchell
Sabananthan, Archcna
Zeng, Xianyu
Gilbert, Gwendolyn L.
机构
[1] Westmead Hosp, ICPMR, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia
[2] Univ Technol Sydney, Fac Sci, Dept Cell & Mol Biol, Sydney, NSW 2007, Australia
[3] Univ New S Wales, Sch Biotechnol & Biomol Sci, Kensington, NSW 2033, Australia
[4] Wuhan First Hosp, Wuhan 430022, Hubei Province, Peoples R China
关键词
D O I
10.1128/JCM.44.5.1887-1891.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 "unknowns." The assay identified all target serotypes, but 11, which cross-read with 1 to 4 nonvaccine serotypes, could he distinguished using serotype-specific antisera.
引用
收藏
页码:1887 / 1891
页数:5
相关论文
共 11 条
[1]   6 NEWLY RECOGNIZED TYPES OF STREPTOCOCCUS-PNEUMONIAE [J].
HENRICHSEN, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (10) :2759-2762
[2]   Typing of Streptococcus pneumoniae: Past, Present, and Future [J].
Henrichsen, Jorgen .
AMERICAN JOURNAL OF MEDICINE, 1999, 107 (01) :50-54
[3]   Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization [J].
Kong, F ;
Ma, L ;
Gilbert, GL .
JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 54 (12) :1133-1138
[4]   A molecular-capsular-type prediction system for 90 Streptococcus pneumoniae serotypes using partial cpsA-cpsB sequencing and wzy- or wzx-specific PCR [J].
Kong, FR ;
Wang, WZ ;
Tao, JN ;
Wang, L ;
Wang, Q ;
Sabananthan, A ;
Gilbert, GL .
JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 54 (04) :351-356
[5]   Using cpsA-cpsB sequence polymorphisms and serotype-/group-specific PCR to predict 51 Streptococcus pneumoniae capsular serotypes [J].
Kong, FR ;
Gilbert, GL .
JOURNAL OF MEDICAL MICROBIOLOGY, 2003, 52 (12) :1047-1058
[6]   Serotype identification of group B streptococci by PCR and sequencing [J].
Kong, FR ;
Gowan, S ;
Martin, D ;
James, G ;
Gilbert, GL .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (01) :216-226
[7]   DIAGNOSIS OF BACTEREMIC PNEUMOCOCCAL PNEUMONIA BY AMPLIFICATION OF PNEUMOLYSIN GENE FRAGMENT IN SERUM [J].
SALO, P ;
ORTQVIST, A ;
LEINONEN, M .
JOURNAL OF INFECTIOUS DISEASES, 1995, 171 (02) :479-482
[8]   GP5+/6+PCR followed by reverse line blot analysis enables rapid and high-throughput identification of human papillomavirus genotypes [J].
van den Brule, AJC ;
Pol, R ;
Fransen-Daalmeijer, N ;
Schouls, LM ;
Meijer, CJLM ;
Snijders, PJF .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (03) :779-787
[9]   Simultaneous detection of nine antibiotic resistance-related genes in Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization assay [J].
Zeng, XY ;
Kong, FR ;
Wang, H ;
Darbar, A ;
Gilbert, GL .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2006, 50 (01) :204-209
[10]   Reverse line blot assay for direct identification of seven streptococcus agalactiae major surface protein antigen genes [J].
Zhao, ZT ;
Kong, FR ;
Gilbert, GL .
CLINICAL AND VACCINE IMMUNOLOGY, 2006, 13 (01) :145-149