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Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes
被引:48
作者:

Kong, Fanrong
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机构: Westmead Hosp, ICPMR, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia

Brown, Mitchell
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机构: Westmead Hosp, ICPMR, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia

Sabananthan, Archcna
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机构: Westmead Hosp, ICPMR, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia

Zeng, Xianyu
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机构: Westmead Hosp, ICPMR, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia

Gilbert, Gwendolyn L.
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机构: Westmead Hosp, ICPMR, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia
机构:
[1] Westmead Hosp, ICPMR, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia
[2] Univ Technol Sydney, Fac Sci, Dept Cell & Mol Biol, Sydney, NSW 2007, Australia
[3] Univ New S Wales, Sch Biotechnol & Biomol Sci, Kensington, NSW 2033, Australia
[4] Wuhan First Hosp, Wuhan 430022, Hubei Province, Peoples R China
关键词:
D O I:
10.1128/JCM.44.5.1887-1891.2006
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 "unknowns." The assay identified all target serotypes, but 11, which cross-read with 1 to 4 nonvaccine serotypes, could he distinguished using serotype-specific antisera.
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页码:1887 / 1891
页数:5
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