The "dispensable" portion of RAG2 is necessary for efficient V-to-DJ rearrangement during B and T cell development

被引：123

作者：

Liang, HE

Hsu, LY

Cado, D

Cowell, LG

Kelsoe, G

Schlissel, MS ^{[1
]}

机构：

[1] Univ Calif Berkeley, Div Immunol, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[2] Johns Hopkins Univ, Sch Med, Grad Program Immunol, Baltimore, MD 21205 USA

[3] Duke Univ, Med Ctr, Dept Immunol, Durham, NC 27710 USA

来源：

IMMUNITY | 2002年 / 17卷 / 05期

关键词：

D O I：

10.1016/S1074-7613(02)00448-X

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Previous in vitro studies defined the minimal regions of RAG1 and RAG2 essential for V(D)J recombination. In order to characterize the role of the C-terminal "dispensable" portion of RAG2, we generated core-RAG2 knock-in mice. We found that the core-RAG2-containing recombinase complex is selectively defective in catalyzing V-to-DJ rearrangement at the IgH and TCRO loci, resulting in partial developmental blocks in B and T lymphopoiesis. Analysis of recombination intermediates showed defects at the cleavage phase of the reaction. We also observed a reduction in overall recombinase activity in core-RAG2-expressing thymocytes, leading us to suggest that the interaction of a defective recombinase with RSS sequences unique to VH and Vbeta gene segments may underlie the specific V-to-DJ rearrangement defect in core-RAG2 mice.

引用

页码：639 / 651

页数：13

共 42 条

[21] THE DEFECT IN MURINE SEVERE COMBINED IMMUNE-DEFICIENCY - JOINING OF SIGNAL SEQUENCES BUT NOT CODING SEGMENTS IN V(D)J RECOMBINATION [J].