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Immobilization of beta-glucosidase using the cellulose-binding domain of Bacillus subtilis endo-beta-1,4-glucanase

被引：9
作者：
Ahn, DH
Kim, H
Pack, MY
机构：
[1] SUNCHON NATL UNIV, DEPT AGR CHEM, SUNCHON 540742, SOUTH KOREA
[2] KAIST, DEPT BIOTECHNOL, TAEJON 305338, SOUTH KOREA
[3] CHEILJEDANG COOPERAT, R&D CTR, INCHON 467810, SOUTH KOREA
来源：
BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 05期
关键词：
D O I：
10.1023/A:1018360530691
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A recombinant plasmid p beta CBD was constructed for immobilization of Cellulomonas fimi beta-glucosidase (Cbg) using the cellulose-binding domain (CBD) of Bacillus subtilis BSE 616 endo-beta-1,4-glucanase (Beg). The Cbg-CBDBeg fusion protein, 80 kDa, was expressed in Escherichia coli and immobilized to Avicel. Cellobiose was completely hydrolyzed with the immobilized fusion protein. The fusion protein bound to Avicel retained full activity during continuous operation for 24 h at 4 degrees C.
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收藏
页码：483 / 486
页数：4
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