A new family of plant transcription factors displays a novel ssDNA-binding surface

被引：78

作者：

Desveaux, D ^{[1
]}

Allard, J ^{[1
]}

Brisson, N ^{[1
]}

Sygusch, J ^{[1
]}

机构：

[1] Univ Montreal, Dept Biochem, Montreal, PQ H3C 3J7, Canada

来源：

NATURE STRUCTURAL BIOLOGY | 2002年 / 9卷 / 07期

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

D O I：

10.1038/nsb814

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of p24, the single-stranded DNA (ssDNA) binding subunit of the plant defense transcription factor PBF-2, has been determined to 2.3 Angstrom resolution. p24 is representative of a novel family of ubiquitous plant-specific proteins that we refer to as the Whirly family because of their quaternary structure. PBF-2 is composed of four p24 molecules that interact through a helix-loop-helix motif. This interaction produces a central pore, with beta-strands radiating outwards, resulting in a whirligig appearance to the quaternary structure. The noncrystallographic C-4 symmetry arrangement of p24 subunits is novel for ssDNA binding proteins and may explain the binding specificity of PBF-2. This structural arrangement also supports the role of PBF-2 in binding melted promoter regions to modulate gene expression.

引用

页码：512 / 517

页数：6

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