Purification of papain from Carica papaya latex:: Aqueous two-phase extraction versus two-step salt precipitation

被引：173

作者：

Nitsawang, Sarote

Hatti-Kaul, Rajni ^{[1
]}

Kanasawuda, Pawinee

机构：

[1] Lund Univ, Ctr Chem & Chem Engn, Dept Biotechnol, SE-22100 Lund, Sweden

[2] Chiang Mai Univ, Fac Sci, Dept Chem, Chiang Mai 50200, Thailand

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2006年 / 39卷 / 05期

关键词：

Carica papaya latex; papain; aqueous two-phase extraction; two-step salt precipitation;

D O I：

10.1016/j.enzmictec.2006.02.013

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dependent on the initial protein concentration in the material used for processing. Highly pure papain was obtained in a much shorter processing time directly from unclarified latex with the use of an aqueous two-phase system consisting of 8% (w/w) polyethylene glycol and 15% (w/w) ammonium sulfate. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：1103 / 1107

页数：5

共 25 条

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