Electrochemical real-time polymerase chain reaction

被引:75
作者
Yeung, Stephen S. W.
Lee, Thomas M. H.
Hsing, I-Ming [1 ]
机构
[1] Hong Kong Univ Sci & Technol, Dept Chem Engn, Kowloon, Hong Kong, Peoples R China
[2] Hong Kong Univ Sci & Technol, Bioengn Program, Kowloon, Hong Kong, Peoples R China
关键词
DNA; PCR; AMPLIFICATION; BIOSENSORS;
D O I
10.1021/ja065733j
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In this work, we report the first electrochemistry-based real-time polymerase chain reaction technique for sequence-specific nucleic acid detection. This new technique builds upon the advantages of the well-established fluorescence-based counterpart, such as short assay time (simultaneous target DNA amplification and detection). In addition, this electrochemical approach could employ simple and miniaturizable instrumentation compared to the bulky and expensive optics required in the fluorescence-based schemes. We have demonstrated a proof-of-concept experiment showing that the utilization of solid-phase extension of the electrode surface-immobilized capture probe with Fc-dUTP during PCR resulted in the accumulation of the redox marker on the transducer surface. This new technique can be applied to a microfabricated PCR electrochemical device for point-of-care diagnostics as well as on-site environmental monitoring and biowarfare agent detection. Copyright © 2006 American Chemical Society.
引用
收藏
页码:13374 / 13375
页数:2
相关论文
共 18 条
  • [1] Enzymatic synthesis of redox-labeled RNA and dual-potential detection at DNA-modified electrodes
    Di Giusto, DA
    Wlassoff, WA
    Giesebrecht, S
    Gooding, JJ
    King, GC
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (21) : 2809 - 2812
  • [2] Multipotential electrochemical detection of primer extension reactions on DNA self-assembled monolayers
    Di Giusto, DA
    Wlassoff, WA
    Giesebrecht, S
    Gooding, JJ
    King, GC
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (13) : 4120 - 4121
  • [3] Dorak MT., 2006, Real-time PCR, P1
  • [4] Electrochemical DNA sensors
    Drummond, TG
    Hill, MG
    Barton, JK
    [J]. NATURE BIOTECHNOLOGY, 2003, 21 (10) : 1192 - 1199
  • [5] Gooding JJ, 2002, ELECTROANAL, V14, P1149, DOI 10.1002/1521-4109(200209)14:17<1149::AID-ELAN1149>3.0.CO
  • [6] 2-8
  • [7] Real time quantitative PCR
    Heid, CA
    Stevens, J
    Livak, KJ
    Williams, PM
    [J]. GENOME RESEARCH, 1996, 6 (10): : 986 - 994
  • [8] SIMULTANEOUS AMPLIFICATION AND DETECTION OF SPECIFIC DNA-SEQUENCES
    HIGUCHI, R
    DOLLINGER, G
    WALSH, PS
    GRIFFITH, R
    [J]. BIO-TECHNOLOGY, 1992, 10 (04): : 413 - 417
  • [9] A versatile toolbox for variable DNA functionalization at high density
    Jäger, S
    Rasched, G
    Kornreich-Leshem, H
    Engeser, M
    Thum, O
    Famulok, M
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (43) : 15071 - 15082
  • [10] Recent trends in electrochemical DNA biosensor technology
    Kerman, K
    Kobayashi, M
    Tamiya, E
    [J]. MEASUREMENT SCIENCE AND TECHNOLOGY, 2004, 15 (02) : R1 - R11