Self-Assembly of Enzymes on DNA Scaffolds: En Route to Biocatalytic Cascades and the Synthesis of Metallic Nanowires

被引：116

作者：

Wilner, Ofer I. ^{[1
]}

Shimron, Simcha ^{[1
]}

Weizmann, Yossi ^{[1
]}

Wang, Zhen-Gang ^{[1
]}

Willner, Itamar ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

来源：

NANO LETTERS | 2009年 / 9卷 / 05期

基金：

以色列科学基金会;

关键词：

NANOSTRUCTURES; PROTEIN; NANOPARTICLES; ARRAYS; NANOBIOTECHNOLOGY; TEMPLATES; MOLECULES;

D O I：

10.1021/nl900302z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

DNA strands consisting of programmed sequence-specific domains were synthesized by the rolling circle amplification (RCA) process. The spatial positioning of glucose oxidase (GOx) and of horseradish peroxidase (HRP) on the RCA-synthesized DNA template via hybridization enabled the activation of the bienzyme cascade. The GOx-catalyzed oxidation of glucose yielded gluconic acid and H2O2, and the resulting H2O2 oxidized 2,2'-azino-bis[3-ethylbenzthiazoline-6-suffonic-acid] (ABTS(2-)) in the presence of HRP. The enzyme cascade could not be activated in the absence of the organizing DNA template or in the presence of a foreign DNA. Also, Au NPs-functionalized GOx was hybridized with the RCA-synthesized single-stranded DNA. The biocatalytic growth of the NPs through the oxidation of glucose, in the presence of AuCl4-, enabled the synthesis of 1-5 mu m long Au wires, exhibiting a width of ca. 150 nm.

引用

页码：2040 / 2043

页数：4

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