Nanoindentation of bone: Comparison of specimens tested in liquid and embedded in polymethylmethacrylate

被引:119
作者
Bushby, AJ [1 ]
Ferguson, VL
Boyde, A
机构
[1] Queen Mary Univ London, Dept Mat, London E1 4NS, England
[2] UCL, Dept Anat & Dev Biol, London WC1E 6BT, England
[3] Queen Mary Univ London, Inst Dent, Ctr Oral Growth & Dev, London E1 2AD, England
关键词
D O I
10.1557/jmr.2004.19.1.249
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Elastic modulus of bone was investigated by nanoindentation using common methods of sample preparation, data collection, and analysis, and compared to dynamic mechanical analysis (DMA: three-point bending) for the same samples. Nanoindentation (Berkovich, 5 mum and 21 mum radii spherical indenters) and DMA were performed on eight wet and dehydrated (100% ethanol), machined equine cortical bone beams. Samples were embedded in polymethylmethacrylate (PMMA) and mechanical tests repeated. Indentation direction was transverse to the bone long axis while DMA tested longitudinally, giving approximately 12% greater :modulus in DMA. For wet samples, nanoindentation with spherical indenters revealed a low modulus surface layer. Estimates of the volume of material contributing to elastic modulus measurement showed that the surface layer influences the measured modulus at low loads. Consistent results were obtained for embedded tissue regardless of indenter Geometry, provided appropriate methods and analysis were used. Modulus increased for nanoindentation +/-21 mum radius indenter) from 11.7 GPa +/-1.7 to 15.0 GPa +/-2.2 to 19.4 GPa 2.1, for wet, dehydrated in ethanol, and embedded conditions, respectively. The large increases in elastic modulus caused by replacing water with ethanol and ethanol with PMMA demonstrate that the role of water in fine pore space and its interaction with collagen strongly influence the mechanical behavior of the tissue.
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页码:249 / 259
页数:11
相关论文
共 38 条
[31]  
OYENTIESMA M, 2001, MAT RES SOC S P, V649
[32]   Effects of drying on the mechanical properties of bovine femur measured by nanoindentation [J].
Rho, JY ;
Pharr, GM .
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 1999, 10 (08) :485-488
[33]   Variations in the individual thick lamellar properties within osteons by nanoindentation [J].
Rho, JY ;
Zioupos, P ;
Currey, JD ;
Pharr, GM .
BONE, 1999, 25 (03) :295-300
[34]  
Rho JY, 2001, J EXP BIOL, V204, P1775
[35]   A C-13 NMR-STUDY ON COLLAGENS IN THE SOLID-STATE - HYDRATION DEHYDRATION-INDUCED CONFORMATIONAL CHANGE OF COLLAGEN AND DETECTION OF INTERNAL MOTIONS [J].
SAITO, H ;
YOKOI, M .
JOURNAL OF BIOCHEMISTRY, 1992, 111 (03) :376-382
[36]   EFFECT OF SUSPENSION ON MOUSE BONE MICROHARDNESS [J].
SIMSKE, SJ ;
BROZ, JJ ;
LUTTGES, MW .
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 1995, 6 (08) :486-491
[37]   The elastic properties of trabecular and cortical bone tissues are similar: results from two microscopic measurement techniques [J].
Turner, CH ;
Rho, J ;
Takano, Y ;
Tsui, TY ;
Pharr, GM .
JOURNAL OF BIOMECHANICS, 1999, 32 (04) :437-441
[38]   Elastic modulus and hardness of cortical and trabecular bone lamellae measured by nanoindentation in the human femur [J].
Zysset, PK ;
Guo, XE ;
Hoffler, CE ;
Moore, KE ;
Goldstein, SA .
JOURNAL OF BIOMECHANICS, 1999, 32 (10) :1005-1012