Structure of an acidic exopolysaccharide of Burkholderia pseudomallei

被引：50

作者：

Nimtz, M

Wray, V

Domke, T

Brenneke, B

Haussler, S

Steinmetz, I

机构：

[1] HANNOVER MED SCH, INST MED MICROBIOL, D-30625 HANNOVER, GERMANY

[2] GESELL BIOTECHNOL FORSCH MBH, D-3300 BRAUNSCHWEIG, GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1997年 / 250卷 / 02期

关键词：

Burkholderia pseudomallei; exopolysaccharide;

D O I：

10.1111/j.1432-1033.1997.0608a.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A recently described water-soluble exopolysaccharide of Burkholderia pseudomallei recognized by the IgG 1 monoclonal antibody 3015 [Steinmetz, I., Rohde, M. & Brenneke, B. (1995) Infect. Immun. 63. 3959-3965] was isolated by repetitive ethanol-precipitation steps and by anion-exchange chromatography. The structure of the polysaccharide was determined by a combination of chemical-derivatization and mass-spectrometric techniques (compositional and methylation analysis, GC/MS, and electrospray-ionization-MS/MS of reduced and permethylated hydrolytic fragments), and two-dimensional H-1-NMR methods (COSY, TOCSY and NOESY) and confirmed by isolation and structural characterization of the depolymerized repeating unit of the polysaccharide. The combined structural data established a linear tetrasaccharide repeating unit consisting of three galactose residues, one bearing a 2-linked O-acetyl group, and a 3-deoxy-D-manno-2-octulosonic acid residue.

引用

页码：608 / 616

页数：9

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