A modular tyrosine kinase deoxyribozyme with discrete aptamer and catalyst domains

被引：9

作者：

Dokukin, Victor ^{[1
]}

Silverman, Scott K. ^{[1
]}

机构：

[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 66期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

IN-VITRO EVOLUTION; ROLLING CIRCLE AMPLIFICATION; PHOSPHORYLATING DNA; SIGNALING APTAMERS; RNA; RIBOZYME; ENZYMES; SENSORS; ATP; SPECIFICITY;

D O I：

10.1039/c4cc04253k

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

We assess the utility of integrating a predetermined aptamer DNA module adjacent to a random catalytic DNA region for identifying new deoxyribozymes by in vitro selection. By placing a known ATP aptamer next to an N-40 random region, an explicitly modular DNA catalyst for tyrosine side chain phosphotylation is identified. The results have implications for broader identification of deoxyribozymes that function with small-molecule substrates.

引用

收藏

页码：9317 / 9320

页数：4

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