Potential protein partners for the human TIMAP revealed by bacterial two-hybrid screening

被引：15

作者：

Adyshev, Djanybek M. ^{[1
]}

Kolosova, Irina A. ^{[1
]}

Verin, Alexander D. ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Med, Div Pulm & Crit Care Med, Baltimore, MD 21224 USA

来源：

MOLECULAR BIOLOGY REPORTS | 2006年 / 33卷 / 02期

关键词：

bacterioMatch two-hybrid system; cytoskeleton; partner proteins; TIMAP;

D O I：

10.1007/s11033-005-2311-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

BacterioMatch Two-Hybrid System (Stratagene) was applied in order to identify potential human TIMAP interaction proteins in the lung. TIMAP highly expressed in endothelial cells and may be involved in endothelial cytoskeletal and barrier regulation. Seven TIMAP interacting partner proteins were identified. Four of identified proteins: cystein and glycine-rich protein 1, eukaryotic translation elongation factor 2, U5 snRNP-specific protein 116 kD, and solute carrier family 3 member 2 are involved in actin cytoskeleton organization, cell adhesion or translation and transcriptional regulation.

引用

页码：83 / 89

页数：7

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