Endocytosis of anti-CCK-B/gastrin receptor antibody and effect on hepatoma cell lines

被引：6

作者：

Stubbs, M

Khan, K

Watson, SA

Savage, K

Dhillon, AP

Caplin, ME

机构：

[1] UCL Royal Free & Univ Coll Med Sch, Dept Histopathol, London, England

[2] UCL Royal Free & Univ Coll Med Sch, Dept Med, London, England

[3] Univ Nottingham, Queens Med Ctr, Canc Studies Unit, Nottingham NG7 2RD, England

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2002年 / 50卷 / 09期

关键词：

gastrin; CCK-B receptor; endocytosis; apoptosis; hepatocyte; tumor;

D O I：

10.1177/002215540205000908

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Immunotherapy has considerable potential in the treatment of cancer. Here we report on the uptake of an antibody raised against the CCK-B/Gastrin receptor (CCK-BR) by liver embryonic and liver tumor cell lines. In all five cell lines studied, expression of CCK-BR and uptake of labeled anti-CCK-BR antibody was observed. The labeled anti-CCK-BR antibody was localized in both the cytoplasm and nucleus of cells. In addition, we found a coincidence between the uptake of the labeled antibody by cells and the occurrence of apoptosis (cell death). The results suggest that antibodies directed against CCK-BR have potential for targeting and possibly destroying tumor cells bearing the receptor.

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页码：1213 / 1217

页数：5

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