Parallel detection of antigen-specific T-cell responses by multidimensional encoding of MHC multimers

被引:229
作者
Hadrup, Sine Reker [1 ]
Bakker, Arnold H. [1 ]
Shu, Chengyi J. [1 ]
Andersen, Rikke S. [2 ]
van Veluw, Jerre [1 ]
Hombrink, Pleun [3 ]
Castermans, Emilie [1 ]
Straten, Per thor [2 ]
Blank, Christian [1 ]
Haanen, John B. [1 ]
Heemskerk, Mirjam H. [3 ]
Schumacher, Ton N. [1 ]
机构
[1] Netherlands Canc Inst, Div Immunol, NL-1066 CX Amsterdam, Netherlands
[2] Herlev Univ Hosp, Dept Hematol, Ctr Canc Immune Therapy, DK-2730 Herlev, Denmark
[3] Leiden Univ, Med Ctr, Dept Hematol, Leiden, Netherlands
关键词
CLASS-I LIGANDS; EPITOPES; IDENTIFICATION; LYMPHOCYTES; TECHNOLOGY; COMPLEXES; PHENOTYPE; MOLECULES; EXCHANGE; HLA-A1;
D O I
10.1038/nmeth.1345
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The use of fluorescently labeled major histocompatibility complex multimers has become an essential technique for analyzing disease- and therapy-induced T-cell immunity. Whereas classical major histocompatibility complex multimer analyses are well-suited for the detection of immune responses to a few epitopes, limitations on human-subject sample size preclude a comprehensive analysis of T-cell immunity. To address this issue, we developed a combinatorial encoding strategy that allows the parallel detection of a multitude of different T-cell populations in a single sample. Detection of T cells from peripheral blood by combinatorial encoding is as efficient as detection with conventionally labeled multimers but results in a substantially increased sensitivity and, most notably, allows comprehensive screens to be performed. We obtained proof of principle for the feasibility of large-scale screening of human material by analysis of human leukocyte antigen A3- restricted T-cell responses to known and potential melanoma-associated antigens in peripheral blood from individuals with melanoma.
引用
收藏
页码:520 / U79
页数:9
相关论文
共 20 条
[1]   Phenotypic analysis of antigen-specific T lymphocytes [J].
Altman, JD ;
Moss, PAH ;
Goulder, PJR ;
Barouch, DH ;
McHeyzerWilliams, MG ;
Bell, JI ;
McMichael, AJ ;
Davis, MM .
SCIENCE, 1996, 274 (5284) :94-96
[2]  
[Anonymous], BIOMETRICS
[3]   Memory CD8+ T cells vary in differentiation phenotype in different persistent virus infections [J].
Appay, V ;
Dunbar, PR ;
Callan, M ;
Klenerman, P ;
Gillespie, GMA ;
Papagno, L ;
Ogg, GS ;
King, A ;
Lechner, F ;
Spina, CA ;
Little, S ;
Havlir, DV ;
Richman, DD ;
Gruener, N ;
Pape, G ;
Waters, A ;
Easterbrook, P ;
Salio, M ;
Cerundolo, V ;
McMichael, AJ ;
Rowland-Jones, SL .
NATURE MEDICINE, 2002, 8 (04) :379-385
[4]   Phenotype and Function of Human T Lymphocyte Subsets: Consensus and Issues [J].
Appay, Victor ;
van Lier, Rene A. W. ;
Sallusto, Federica ;
Roederer, Mario .
CYTOMETRY PART A, 2008, 73A (11) :975-983
[5]   A direct estimate of the human αβ T cell receptor diversity [J].
Arstila, TP ;
Casrouge, A ;
Baron, V ;
Even, J ;
Kanellopoulos, J ;
Kourilsky, P .
SCIENCE, 1999, 286 (5441) :958-961
[6]   MHC multimer technology: current status and future prospects [J].
Bakker, AH ;
Schumacher, TNM .
CURRENT OPINION IN IMMUNOLOGY, 2005, 17 (04) :428-433
[7]   Conditional MHC class I ligands and peptide exchange technology for the human MHC gene products HLA-A1, -A3, -A11 and -B7 [J].
Bakker, Arnold H. ;
Hoppes, Rieuwert ;
Linnemann, Carsten ;
Toebes, Mireille ;
Rodenko, Boris ;
Berkers, Celia R. ;
Hadrup, Sine Reker ;
van Esch, Wirn J. E. ;
Heemskerk, Mirjam H. M. ;
Ovaa, Huib ;
Schumacher, Ton N. M. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (10) :3825-3830
[8]   Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry [J].
Chattopadhyay, Pratip K. ;
Price, David A. ;
Harper, Theresa F. ;
Betts, Michael R. ;
Yu, Joanne ;
Gostick, Emma ;
Perfetto, Stephen P. ;
Goepfert, Paul ;
Koup, Richard A. ;
De Rosa, Stephen C. ;
Bruchez, Marcel P. ;
Roederer, Mario .
NATURE MEDICINE, 2006, 12 (08) :972-977
[9]   Parasite Stage-Specific Recognition of Endogenous Toxoplasma gondii-Derived CD8+ T Cell Epitopes [J].
Frickel, Eva-Maria ;
Sahoo, Nivedita ;
Hopp, Johnathan ;
Gubbels, Marc-Jan ;
Craver, Mary Patricia J. ;
Knoll, Laura J. ;
Ploegh, Hidde L. ;
Grotenbreg, Gijsbert M. .
JOURNAL OF INFECTIOUS DISEASES, 2008, 198 (11) :1625-1633
[10]   HLA-A2-PEPTIDE COMPLEXES - REFOLDING AND CRYSTALLIZATION OF MOLECULES EXPRESSED IN ESCHERICHIA-COLI AND COMPLEXED WITH SINGLE ANTIGENIC PEPTIDES [J].
GARBOCZI, DN ;
HUNG, DT ;
WILEY, DC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (08) :3429-3433