The use of fluorescently labeled major histocompatibility complex multimers has become an essential technique for analyzing disease- and therapy-induced T-cell immunity. Whereas classical major histocompatibility complex multimer analyses are well-suited for the detection of immune responses to a few epitopes, limitations on human-subject sample size preclude a comprehensive analysis of T-cell immunity. To address this issue, we developed a combinatorial encoding strategy that allows the parallel detection of a multitude of different T-cell populations in a single sample. Detection of T cells from peripheral blood by combinatorial encoding is as efficient as detection with conventionally labeled multimers but results in a substantially increased sensitivity and, most notably, allows comprehensive screens to be performed. We obtained proof of principle for the feasibility of large-scale screening of human material by analysis of human leukocyte antigen A3- restricted T-cell responses to known and potential melanoma-associated antigens in peripheral blood from individuals with melanoma.
机构:
John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, EnglandJohn Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Appay, V
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Dunbar, PR
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Dunbar, PR
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Callan, M
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Callan, M
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Klenerman, P
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Klenerman, P
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Gillespie, GMA
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Gillespie, GMA
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Papagno, L
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Papagno, L
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Ogg, GS
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Ogg, GS
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King, A
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
King, A
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Lechner, F
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Lechner, F
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Spina, CA
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Spina, CA
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Little, S
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Little, S
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Havlir, DV
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Havlir, DV
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Richman, DD
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Richman, DD
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Gruener, N
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Gruener, N
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Pape, G
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Pape, G
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Waters, A
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Waters, A
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Easterbrook, P
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Easterbrook, P
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Salio, M
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Salio, M
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Cerundolo, V
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Cerundolo, V
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McMichael, AJ
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
McMichael, AJ
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Rowland-Jones, SL
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机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
机构:
Univ Amsterdam, Acad Med Ctr, Dept Expt Immunol, NL-1105 AZ Amsterdam, NetherlandsUniv Paris 06, Hop La Pitie Salpetriere, INSERM,Cellular Immunol Lab, U543,Avenir Grp, F-75013 Paris, France
van Lier, Rene A. W.
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Sallusto, Federica
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h-index: 0
机构:
Inst Res Biomed, CH-6500 Bellinzona, SwitzerlandUniv Paris 06, Hop La Pitie Salpetriere, INSERM,Cellular Immunol Lab, U543,Avenir Grp, F-75013 Paris, France
Sallusto, Federica
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Roederer, Mario
论文数: 0引用数: 0
h-index: 0
机构:
NIH, Vaccine Res Ctr, Bethesda, MD 20892 USAUniv Paris 06, Hop La Pitie Salpetriere, INSERM,Cellular Immunol Lab, U543,Avenir Grp, F-75013 Paris, France
机构:
John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, EnglandJohn Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Appay, V
;
Dunbar, PR
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Dunbar, PR
;
Callan, M
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Callan, M
;
Klenerman, P
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Klenerman, P
;
Gillespie, GMA
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Gillespie, GMA
;
Papagno, L
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Papagno, L
;
Ogg, GS
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Ogg, GS
;
King, A
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
King, A
;
Lechner, F
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Lechner, F
;
Spina, CA
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Spina, CA
;
Little, S
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Little, S
;
Havlir, DV
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Havlir, DV
;
Richman, DD
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Richman, DD
;
Gruener, N
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Gruener, N
;
Pape, G
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Pape, G
;
Waters, A
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Waters, A
;
Easterbrook, P
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Easterbrook, P
;
Salio, M
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Salio, M
;
Cerundolo, V
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
Cerundolo, V
;
McMichael, AJ
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
McMichael, AJ
;
Rowland-Jones, SL
论文数: 0引用数: 0
h-index: 0
机构:John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DU, England
机构:
Univ Amsterdam, Acad Med Ctr, Dept Expt Immunol, NL-1105 AZ Amsterdam, NetherlandsUniv Paris 06, Hop La Pitie Salpetriere, INSERM,Cellular Immunol Lab, U543,Avenir Grp, F-75013 Paris, France
van Lier, Rene A. W.
;
Sallusto, Federica
论文数: 0引用数: 0
h-index: 0
机构:
Inst Res Biomed, CH-6500 Bellinzona, SwitzerlandUniv Paris 06, Hop La Pitie Salpetriere, INSERM,Cellular Immunol Lab, U543,Avenir Grp, F-75013 Paris, France
Sallusto, Federica
;
Roederer, Mario
论文数: 0引用数: 0
h-index: 0
机构:
NIH, Vaccine Res Ctr, Bethesda, MD 20892 USAUniv Paris 06, Hop La Pitie Salpetriere, INSERM,Cellular Immunol Lab, U543,Avenir Grp, F-75013 Paris, France