CdiA from Enterobacter cloacae Delivers a Toxic Ribosomal RNase into Target Bacteria

被引：61

作者：

Beck, Christina M. ^{[1
]}

Morse, Robert P. ^{[2
]}

Cunningham, David A. ^{[1
]}

Iniguez, Angelina ^{[2
]}

Low, David A. ^{[1
,3
]}

Goulding, Celia W. ^{[2
,4
]}

Hayes, Christopher S. ^{[1
,3
]}

机构：

[1] Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA

[2] Univ Calif Irvine, Dept Mol Biol & Biochem, Irvine, CA 92697 USA

[3] Univ Calif Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA 93106 USA

[4] Univ Calif Irvine, Dept Pharmaceut Sci, Irvine, CA 92697 USA

来源：

STRUCTURE | 2014年 / 22卷 / 05期

基金：

美国国家卫生研究院;

关键词：

DEPENDENT GROWTH-INHIBITION; VI SECRETION SYSTEM; ERWINIA-CHRYSANTHEMI EC16; ESCHERICHIA-COLI; POSITIVE SELECTION; CRYSTAL-STRUCTURE; CYTOTOXIC DOMAIN; STRUCTURAL BASIS; COLICIN-E3; IMMUNITY;

D O I：

10.1016/j.str.2014.02.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Contact-dependent growth inhibition (CDI) is one mechanism of inter-bacterial competition. CDI+ cells export large CdiA effector proteins, which carry a variety of C-terminal toxin domains (CdiA-CT). CdiA-CT toxins are specifically neutralized by cognate CdiA immunity proteins to protect toxin-producing cells from autoinhibition. Here, we use structure determination to elucidate the activity of a CDI toxin from Enterobacter cloacae (ECL). The structure of CdiA-CT (ECL) resembles the C-terminal nuclease domain of colicin E3, which cleaves 16S ribosomal RNA to disrupt protein synthesis. In accord with this structural homology, we show that CdiA-CT (ECL) uses the same nuclease activity to inhibit bacterial growth. Surprisingly, although colicin E3 and CdiA(ECL) carry equivalent toxin domains, the corresponding immunity proteins are unrelated in sequence, structure, and toxin-binding site. Together, these findings reveal unexpected diversity among 16S rRNases and suggest that these nucleases are robust and versatile payloads for a variety of toxin-delivery platforms.

引用

页码：707 / 718

页数：12

共 46 条

[1] Contact-dependent inhibition of growth in Escherichia coli [J].

Aoki, SK ;

Pamma, R ;

Hernday, AD ;

Bickham, JE ;

Braaten, BA ;

Low, DA .

SCIENCE, 2005, 309 (5738) :1245-1248

[2] Contact-dependent growth inhibition requires the essential outer membrane protein BamA (YaeT) as the receptor and the inner membrane transport protein AcrB [J].

Aoki, Stephanie K. ;

Malinverni, Juliana C. ;

Jacoby, Kyle ;

Thomas, Benjamin ;

Pamma, Rupinderjit ;

Trinh, Brooke N. ;

Remers, Susan ;

Webb, Julia ;

Braaten, Bruce A. ;

Silhavy, Thomas J. ;

Low, David A. .

MOLECULAR MICROBIOLOGY, 2008, 70 (02) :323-340

[3] A widespread family of polymorphic contact-dependent toxin delivery systems in bacteria [J].

Aoki, Stephanie K. ;

Diner, Elie J. ;

de Roodenbeke, Claire t'Kint ;

Burgess, Brandt R. ;

Poole, Stephen J. ;

Braaten, Bruce A. ;

Jones, Allison M. ;

Webb, Julia S. ;

Hayes, Christopher S. ;

Cotter, Peggy A. ;

Low, David A. .

NATURE, 2010, 468 (7322) :439-442

[4] Identification and characterization of RsmE, the founding member of a new RNA base methyltransferase family [J].

Basturea, GN ;

Rudd, KE ;

Deutscher, MP .

RNA, 2006, 12 (03) :426-434

[5] SPECIFIC INACTIVATION OF 16S RIBOSOMAL RNA INDUCED BY COLICIN E3 IN-VIVO [J].

BOWMAN, CM ;

DAHLBERG, JE ;

IKEMURA, T ;

KONISKY, J ;

NOMURA, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (05) :964-&

[6] Inhibition of a ribosome-inactivating ribonuclease: the crystal structure of the cytotoxic domain of colicin E3 in complex with its immunity protein [J].

Carr, S ;

Walker, D ;

James, R ;

Kleanthous, C ;

Hemmings, AM .

STRUCTURE, 2000, 8 (09) :949-960

[7] Colicin biology [J].

Cascales, Eric ;

Buchanan, Susan K. ;

Duche, Denis ;

Kleanthous, Colin ;

Lloubes, Roland ;

Postle, Kathleen ;

Riley, Margaret ;

Slatin, Stephen ;

Cavard, Daniele .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2007, 71 (01) :158-229

[8] STRUCTURED HABITATS AND THE EVOLUTION OF ANTICOMPETITOR TOXINS IN BACTERIA [J].

CHAO, L ;

LEVIN, BR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (10) :6324-6328

[9] Chemical warfare between microbes promotes biodiversity [J].

Czárán, TL ;

Hoekstra, RF ;

Pagie, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (02) :786-790

[10] Whirly transcription factors:: defense gene regulation and beyond [J].

Desveaux, D ;

Maréchal, A ;

Brisson, N .

TRENDS IN PLANT SCIENCE, 2005, 10 (02) :95-102

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