Regulation of nitrate reductase by non-modifiable derivatives of PII in the cells of Synechococcus elongatus strain PCC 7942

被引：11

作者：

Takatani, Nobuyuki ^{[1
]}

Kobayashi, Masaki ^{[1
]}

Maeda, Shin-ichi ^{[1
]}

Omata, Tatsuo ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Lab Mol Plant Physiol, Nagoya, Aichi 4648601, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2006年 / 47卷 / 08期

关键词：

cyanobacterium; nitrate assimilation; nitrate reductase; PII protein; post-translational regulation; Synechococcus elongatus strain PCC 7942;

D O I：

10.1093/pcp/pcj088

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In Synechococcus elongatus, the PII protein inhibits both transport and reduction of nitrate when ammonium is present in the medium. Using a transporter mutant having ammonium-resistant nitrate transport activity as the genetic background, we analyzed specific effects of PII on in vivo nitrate reductase activity by measuring uptake of nitrate from the medium. The results showed that the regulation of nitrate reductase does not require changes in the electric charge or size of the side chain at the phosphorylation site of PII. Phosphorylation of PII is thus unlikely to play a role in the regulation of nitrate reductase.

引用

页码：1182 / 1186

页数：5

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