Validation of random amplified polymorphic DNA assays by ribotyping as tools for epidemiological surveys of Pasteurella from animals

被引：38

作者：

ChaslusDancla, E ^{[1
]}

LesageDescauses, MC ^{[1
]}

LeroySetrin, S ^{[1
]}

Martel, JL ^{[1
]}

Coudert, P ^{[1
]}

Lafont, JP ^{[1
]}

机构：

[1] CTR NATL ETUD VET & ALIMENTAIRES, F-63942 LYON, FRANCE

来源：

VETERINARY MICROBIOLOGY | 1996年 / 52卷 / 1-2期

关键词：

Pasteurella; ribotyping; RAPD assays;

D O I：

10.1016/0378-1135(96)00065-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two collections of strains of Pasteurella were studied for epidemiological purposes by ribotyping and random amplified polymorphic DNA (RAPD) assays. These strains were isolated through two different structures of animal productions: cattle and rabbit. Forty strains of P. haemolytica from cattle reared in independent breeding-herds belonged to only 3 ribotypes after digestion with HindIII and PvuII. No further discrimination of these strains was obtained by RAPD assays. All these 40 strains showed more than 90% of similarity. This result was consistent with the hypothesis of a clonal dissemination of these strains in bovine herds, possibly favoured by the large use of antibiotics. Forty-one strains of P. multocida were isolated in rabbits flocks belonging to 16 breeders. Six of these were linked by commercial relationships. Twenty-eight out of the 29 strains isolated through this commercial network belonged to only three ribotypes whereas the 12 strains from independant breeders belonged to 9 ribotypes. Results of RAPD assays were in accordance with those of ribotyping and validate the use of RAPD assays for epidemiological studies of Pasteurella strains.

引用

页码：91 / 102

页数：12

共 23 条

[11]

MARTEL JL, 1985, RECL MED VET, V161, P1123

[12] BACTERIAL-RESISTANCE MONITORING IN ANIMALS - THE FRENCH NATIONAL EXPERIENCES OF SURVEILLANCE SCHEMES [J].

MARTEL, JL ;

COUDERT, M .

VETERINARY MICROBIOLOGY, 1993, 35 (3-4) :321-338

[13] RESTRICTION-ENDONUCLEASE ANALYSIS AND RIBOTYPING DIFFERENTIATE PASTEURELLA-HAEMOLYTICA SEROTYPE-A1 ISOLATES FROM CATTLE WITHIN A FEEDLOT [J].

MURPHY, GL ;

ROBINSON, LC ;

BURROWS, GE .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (09) :2303-2308

[14] A SIMPLE, SENSITIVE METHOD OF ANALYZING BACTERIAL RIBOSOMAL DNA POLYMORPHISM [J].

PICARDPASQUIER, N ;

OUAGUED, M ;

PICARD, B ;

GOULLET, P ;

KRISHNAMOORTHY, R .

ELECTROPHORESIS, 1989, 10 (03) :186-189

[15]

Rideaud P., 1992, Journal of Applied Rabbit Research, V15, P1389

[16] COMPARING DIFFERENT ISOLATES OF PASTEURELLA-HAEMOLYTICA FROM BEEF-CALVES USING THEIR INVITRO ANTIMICROBIAL SENSITIVITY PATTERNS [J].

SHOO, MK .

VETERINARY MICROBIOLOGY, 1989, 20 (01) :73-78

[17] USE OF AN RIBOSOMAL-RNA PROBE AND RESTRICTION ENDONUCLEASE ANALYSIS TO FINGERPRINT PASTEURELLA-MULTOCIDA ISOLATED FROM TURKEYS AND WILDLIFE [J].

SNIPES, KP ;

HIRSH, DC ;

KASTEN, RW ;

HANSEN, LM ;

HIRD, DW ;

CARPENTER, TE ;

MCCAPES, RH .

JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (08) :1847-1853

[18] USING RIBOSOMAL-RNA GENE RESTRICTION PATTERNS IN DISTINGUISHING ISOLATES OF PASTEURELLA-HAEMOLYTICA FROM BIGHORN SHEEP (OVIS-CANADENSIS) [J].

SNIPES, KP ;

KASTEN, RW ;

WILD, MA ;

MILLER, MW ;

JESSUP, DA ;

SILFLOW, RL ;

FOREYT, WJ ;

CARPENTER, TE .

JOURNAL OF WILDLIFE DISEASES, 1992, 28 (03) :347-354

[19] FINGERPRINTING GENOMES USING PCR WITH ARBITRARY PRIMERS [J].

WELSH, J ;

MCCLELLAND, M .

NUCLEIC ACIDS RESEARCH, 1990, 18 (24) :7213-7218

[20] DNA POLYMORPHISMS AMPLIFIED BY ARBITRARY PRIMERS ARE USEFUL AS GENETIC-MARKERS [J].

WILLIAMS, JGK ;

KUBELIK, AR ;

LIVAK, KJ ;

RAFALSKI, JA ;

TINGEY, SV .

NUCLEIC ACIDS RESEARCH, 1990, 18 (22) :6531-6535

← 1 2 3 →