Simple and rapid process for single cell micro-patterning

被引：193

作者：

Azioune, Ammar ^{[1
]}

Storch, Marko ^{[1
]}

Bornens, Michel ^{[1
]}

Thery, Manuel ^{[2
]}

Piel, Matthieu ^{[1
]}

机构：

[1] Inst Curie, CNRS, UMR144, F-75005 Paris, France

[2] CEA, DSV, DRDC, Lab Biopuces, F-38054 Grenoble, France

来源：

LAB ON A CHIP | 2009年 / 9卷 / 11期

关键词：

POLYMER SURFACES; ADHESION; ORIENTATION;

D O I：

10.1039/b821581m

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a simple and environmentally friendly process for cell patterning on glass covered with an ultrathin layer of poly-L-lysine-grafted-polyethylene glycol (PLL-g-PEG) by exposure to deep UV light. The patterned substrates are stable for months in the lab atmosphere before incubation with proteins. Incubation with proteins resulted in well defined patterns, with high feature resolution. RPE-1 cells seeded on fibronectin/fibrinogen-Alexa 488 patterns were constrained for days on the deep UV exposed regions. Finally, large glass plates were patterned with high homogeneity enabling the assembly of micro-patterned microplates in 96-well format.

引用

收藏

页码：1640 / 1642

页数：3

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