Investigation of living pancreas tumor cells by digital holographic microscopy

被引：321

作者：

Kemper, Bjorn

Carl, Daniel

Schnekenburger, Jurgen

Bredebusch, Ilona

Schaefer, Marcus

Domschke, Wolfram

von Bally, Gert

机构：

[1] Univ Munster, Biophys Lab, D-48129 Munster, Germany

[2] Univ Munster, Dept Med B, D-48129 Munster, Germany

[3] Univ Munster, Dept Phys, D-48129 Munster, Germany

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2006年 / 11卷 / 03期

关键词：

digital holography; microscopy; marker-free dynamic single cell analysis;

D O I：

10.1117/1.2204609

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Digital holographic microscopy provides new facilities for contactless and marker-free quantitative phase contrast imaging. In this work, a digital holographic microscopy method for the integral refractive index determination of living single cells in cell culture medium is presented. Further, the obtained refractive index information is applied to full field thickness and shape determination of adherent pancreas tumor cells, as well as for analysis of drug-induced dynamic changes of a single cell's cytoskeleton. The results demonstrate that digital holographic microscopy is a quantitative phase contrast technique for living cells under conventional laboratory conditions. (c) 2006 Society of Photo-Optical Instrumentation Engineers.

引用

页数：8

共 32 条

[21] Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy [J].