Effects of specimen refractive index on confocal imaging

被引：89

作者：

Sheppard, CJR

Torok, P

机构：

[1] UNIV SYDNEY,AUSTRALIAN KEY CTR MICROSCOPY & MICROANAL,SYDNEY,NSW 2006,AUSTRALIA

[2] UNIV CAMBRIDGE,MULTIIMAGING CTR,CAMBRIDGE CB2 3DY,ENGLAND

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1997年 / 185卷

关键词：

aberration; confocal microscopy; point spread function;

D O I：

10.1046/j.1365-2818.1997.d01-627.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The aberrations introduced when focusing within a specimen with a refractive index equal to that of water using an oil-immersion objective are investigated theoretically. The peak intensity in the confocal point spread function drops by a factor of two for focusing less than 10 mu m into the specimen. The effects on scaling of dimensions in the resulting images are discussed. The image exhibits an axial stretching by a factor of about 1.12.

引用

页码：366 / 374

页数：9

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