A highly efficient chemoenzymatic approach toward glycoprotein synthesis

被引：88

作者：

Li, Bing ^{[1
]}

Song, Haijing ^{[1
]}

Hauser, Steven ^{[1
]}

Wang, Lai-Xi ^{[1
]}

机构：

[1] Univ Maryland, Inst Biotechnol, Inst Human Virol, Baltimore, MD 21201 USA

来源：

ORGANIC LETTERS | 2006年 / 8卷 / 14期

关键词：

D O I：

10.1021/ol061056m

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A highly efficient endoglycosidase-catalyzed synthesis of homogeneous glycoproteins is described. By using ribonuclease B as a model system, it was demonstrated that Endo-A could efficiently attach a preassembled oligosaccharide to a GlcNAc-containing protein in a regioand stereospecific manner, when the corresponding sugar oxazoline was used as the donor substrate. The method allows the synthesis of both natural and tailor-made N-linked glycoproteins in excellent yield.

引用

页码：3081 / 3084

页数：4

共 38 条

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