Glycosidase-catalysed synthesis of mannobioses by the reverse hydrolysis activity of α-mannosidase:: partial purification of α-mannosidases from almond meal, limpets and Aspergillus niger

被引：15

作者：

Singh, S ^{[1
]}

Scigelova, M ^{[1
]}

Crout, DHG ^{[1
]}

机构：

[1] Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England

来源：

TETRAHEDRON-ASYMMETRY | 2000年 / 11卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1016/S0957-4166(99)00532-7

中图分类号：

O61 [无机化学];

学科分类号：

070301 [无机化学]; 081704 [应用化学];

摘要：

Two disaccharides, alpha-D-Manp-(1-->2)-D-Manp 6 and alpha-D-Manp-(1-->3)-D-Manp 7, were synthesised from mannose using the reverse hydrolysis activity of the partially purified alpha-mannosidases from almond (Prunus amygdalus) meal and limpets (Patella vulgata). Both disaccharides were isolated by carbon-Celite chromatography. Attempts were also made towards the synthesis of core pentasaccharide using the purified alpha-mannosidase from Aspergillus niger. (C) 2000 Published by Elsevier Science Ltd. All rights reserved.

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收藏

页码：223 / 229

页数：7

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