Expression of a synthetic gene encoding the sweet-tasting protein thaumatin in Escherichia coli

被引:34
作者
Faus, I [1 ]
Patino, C [1 ]
delRio, JL [1 ]
delMoral, C [1 ]
Barroso, HS [1 ]
Rubio, V [1 ]
机构
[1] UNIV AUTONOMA MADRID,CSIC,CTR NACL BIOTECNOL,E-28049 MADRID,SPAIN
关键词
D O I
10.1006/bbrc.1996.1767
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A synthetic gene encoding the aminoacid sequence of the sweet-tasting protein thaumatin II has been assembled and expressed in Escherichia coli. Immunoblotting analysis shows that the expressed recombinant thaumatin has the same molecular weight as the protein from its natural source, the plant Thaumatoccocus daniellii Benth. (C) 1996 Academic Press. Inc.
引用
收藏
页码:121 / 127
页数:7
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