Nano-electrospray ionization mass spectrometry:: addressing analytical problems beyond routine

被引：184

作者：

Karas, M ^{[1
]}

Bahr, U ^{[1
]}

Dülcks, T ^{[1
]}

机构：

[1] Goethe Univ Frankfurt, D-60590 Frankfurt, Germany

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 2000年 / 366卷 / 6-7期

关键词：

D O I：

10.1007/s002160051561

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The advent of nano-electrospray ionization (nano-ESI) has considerably extended the usability of ESI in the analytical mass spectrometric laboratory. One of the remarkable features of nano-ESI is its extremely low sample consumption. Only a few microliters of analyte solution (10(-5)-10(-8) M) are sufficient for molecular weight determination and structural investigations by MS/MS. But nano-ESI is more than just a minimized-flow ESI; the low solvent flow rate also affects the mechanism of ion formation. As a consequence, the area of ESI-MS applications is significantly enhanced. Oligosaccharides, glycosides as well as glycoproteins can be analyzed more easily than with normal ion spray. The same holds for the analysis of non-covalent complexes sprayed directly from aqueous solutions.

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页码：669 / 676

页数：8

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