An ordered yeast artificial chromosome library covering over half of rice chromosome 6

被引：12

作者：

Umehara, Y ^{[1
]}

Tanoue, H ^{[1
]}

Kurata, N ^{[1
]}

Ashikawa, I ^{[1
]}

Minobe, Y ^{[1
]}

Sasaki, T ^{[1
]}

机构：

[1] SOC TECHNOINNOVAT AGR FORESTRY & FISHERIES,NATL INST AGROBIOL RESOURCES,RICE GENOME RES PROGRAM,TSUKUBA,IBARAKI 305,JAPAN

来源：

GENOME RESEARCH | 1996年 / 6卷 / 10期

关键词：

D O I：

10.1101/gr.6.10.935

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Yeast artificial chromosome (YAC) clones carrying DNA marker sequences located on the rice genetic map of chromosome 6 were ordered for physical mapping. A total of 122 restriction fragment length polymorphism markers, 16 sequence-tagged site markers, and five random amplified polymorphic DNA markers located, on average, at 0.9-cM intervals, were used for YAC clone screening by colony/Southern hybridization and PCR screening, respectively. A total of 216 individual YACs were selected from our YAC library of 7000 clones covering six genome equivalents. Each DNA marker could select, on average, 4.8 YAC clones, with 11 clones being the maximum. The YACs localized to the corresponding linkage map positions form 43 contigs and encompass about 60% of rice chromosome 6. This is the first step in constructing a physical map covering the whole rice genome by chromosome landing with YAC clones. These YACs and data will be used soon to isolate phenotypical trait genes by map-based cloning.

引用

页码：935 / 942

页数：8

共 25 条

[1] HOMOEOLOGOUS RELATIONSHIPS OF RICE, WHEAT AND MAIZE CHROMOSOMES
AHN, S
ANDERSON, JA
SORRELLS, ME
TANKSLEY, SD
[J]. MOLECULAR & GENERAL GENETICS, 1993, 241 (5-6): : 483 - 490
[2] COMPARATIVE LINKAGE MAPS OF THE RICE AND MAIZE GENOMES
AHN, S
TANKSLEY, SD
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) : 7980 - 7984
[3] Arumuganathan K., 1991, PLANT MOL BIOL REP, V9, P211, DOI DOI 10.1007/BF02672069
[4] CAUSSE MA, 1994, GENETICS, V138, P1251
[5] STABILIZATION OF A YEAST ARTIFICIAL CHROMOSOME CONTAINING PLANT DNA USING A RECOMBINATION-DEFICIENT HOST
DUNFORD, R
VILAGELIU, L
MOORE, G
[J]. PLANT MOLECULAR BIOLOGY, 1993, 21 (06) : 1187 - 1189
[6] CONSERVATION OF FINE-SCALE DNA MARKER ORDER IN THE GENOMES OF RICE AND THE TRITICEAE
DUNFORD, RP
KURATA, N
LAURIE, DA
MONEY, TA
MINOBE, Y
MOORE, G
[J]. NUCLEIC ACIDS RESEARCH, 1995, 23 (14) : 2724 - 2728
[7] SYSTEMATIC SCREENING OF YEAST ARTIFICIAL-CHROMOSOME LIBRARIES BY USE OF THE POLYMERASE CHAIN-REACTION
GREEN, ED
OLSON, MV
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (03) : 1213 - 1217
[8] Hauge B M, 1991, Symp Soc Exp Biol, V45, P45
[9] SEQUENCE-TAGGED SITES (STSS) AS STANDARD LANDMARKERS IN THE RICE GENOME
INOUE, T
ZHONG, HS
MIYAO, A
ASHIKAWA, I
MONNA, L
FUKUOKA, S
MIYADERA, N
NAGAMURA, Y
KURATA, N
SASAKI, T
MINOBE, Y
[J]. THEORETICAL AND APPLIED GENETICS, 1994, 89 (06) : 728 - 734
[10] A SET OF 97 OVERLAPPING YEAST ARTIFICIAL CHROMOSOME CLONES SPANNING THE HUMAN Y-CHROMOSOME EUCHROMATIN
JONES, MH
KHWAJA, OSA
BRIGGS, H
LAMBSON, B
DAVEY, PM
CHALMERS, J
ZHOU, CY
WALKER, EM
ZHANG, Y
TODD, C
FERGUSONSMITH, MA
AFFARA, NA
[J]. GENOMICS, 1994, 24 (02) : 266 - 275

← 1 2 3 →