Synthesis of 2-[(2-pyridyl)amino]ethyl β-D-lactosaminide and evaluation of its acceptor ability for sialyltransferase:: a comparison with 4-methylumbelliferyl and dansyl β-D-lactosaminide

被引：17

作者：

Kajihara, Y

Kamiyama, D

Yamamoto, N

Sakakibara, T

Izumi, M

Hashimoto, H

机构：

[1] Yokohama City Univ, Grad Sch Integrated Sci, Kanazawa Ku, Yokohama, Kanagawa 2360027, Japan

[2] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Life Sci, Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

CARBOHYDRATE RESEARCH | 2004年 / 339卷 / 08期

关键词：

sialyltransferase; enzyme assay; 2-aminopyridyl-tethered oligosaccharide; fluorescence assay; N-acetyl-D-lactosaminide;

D O I：

10.1016/j.carres.2004.03.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the synthesis Of P-D-lactosaminide with a 2-aminopyridyl group that is linked to a glycosyl tether at the reducing end. This fluorescent disaccharide acts as an acceptor for both alpha-(2 --> 6)- and alpha-(2 --> 3)-sialyltransferases. In addition, the acceptor ability of this disaccharide was evaluated and compared with that Of beta-D-lactosaminide having a dansyl or a 4-methylumbelliferyl group. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：1545 / 1550

页数：6

共 18 条

[1] A NEW GENERAL-APPROACH TO FLUOROGENIC ENZYME ASSAYS OF HIGH-SENSITIVITY AND POTENTIAL HIGH SPECIFICITY, ILLUSTRATED BY A PROCEDURE FOR BETA-D-GALACTOSIDASE ESTIMATIONS [J].